V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element

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Standard

V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element. / Roch, F A; Hobi, R; Berchtold, M W; Kuenzle, C C.

I: Nucleic Acids Research, Bind 25, Nr. 12, 1997, s. 2303-10.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Roch, FA, Hobi, R, Berchtold, MW & Kuenzle, CC 1997, 'V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element', Nucleic Acids Research, bind 25, nr. 12, s. 2303-10. <http://nar.oxfordjournals.org/cgi/reprint/25/12/2303>

APA

Roch, F. A., Hobi, R., Berchtold, M. W., & Kuenzle, C. C. (1997). V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element. Nucleic Acids Research, 25(12), 2303-10. http://nar.oxfordjournals.org/cgi/reprint/25/12/2303

Vancouver

Roch FA, Hobi R, Berchtold MW, Kuenzle CC. V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element. Nucleic Acids Research. 1997;25(12):2303-10.

Author

Roch, F A ; Hobi, R ; Berchtold, M W ; Kuenzle, C C. / V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element. I: Nucleic Acids Research. 1997 ; Bind 25, Nr. 12. s. 2303-10.

Bibtex

@article{3fa23d500cb311de8478000ea68e967b,
title = "V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element",
abstract = "The immunoglobulin heavy chain intron enhancer (Emu) not only stimulates transcription but also V(D)J recombination of chromosomally integrated recombination substrates. We aimed at reproducing this effect in recombination competent cells by transient transfection of extrachromosomal substrates. These we prepared by interposing between the recombination signal sequences (RSS) of the plasmid pBlueRec various fragments, including Emu, possibly affecting V(D)J recombination. Our work shows that sequences inserted between RSS 23 and RSS 12, with distances from their proximal ends of 26 and 284 bp respectively, can markedly affect the frequency of V(D)J recombination. We report that the entire Emu, the Emu core as well as its flanking 5' and 3' matrix associated regions (5' and 3' MARs) upregulate V(D)J recombination while the downstream section of the 3' MAR of Emu does not. Also, prokaryotic sequences markedly suppress V(D)J recombination. This confirms previous results obtained with chromosomally integrated substrates, except for the finding that the full length 3' MAR of Emu stimulates V(D)J recombination in an episomal but not in a chromosomal context. The fact that other MARs do not share this activity suggests that the effect is no mediated through attachment of the recombination substrate to a nuclear matrix-associated recombination complex but through cis-activation. The presence of a 26 bp A-T-rich sequence motif in the 5' and 3' MARs of Emu and in all of the other upregulating fragments investigated, leads us to propose that the motif represents a novel recombinational enhancer element distinct from those constituting the Emu core.",
author = "Roch, {F A} and R Hobi and Berchtold, {M W} and Kuenzle, {C C}",
note = "Keywords: Animals; Antibody Diversity; B-Lymphocytes; Base Composition; Base Sequence; Cell Line; Consensus Sequence; Enhancer Elements, Genetic; Gene Rearrangement, B-Lymphocyte; Humans; Immunoglobulin Heavy Chains; Immunoglobulin Joining Region; Immunoglobulin Variable Region; Introns; Mice; Molecular Sequence Data; Protein Sorting Signals; Recombinant Proteins; Recombination, Genetic; Regulatory Sequences, Nucleic Acid; Transcription, Genetic; Transfection",
year = "1997",
language = "English",
volume = "25",
pages = "2303--10",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "12",

}

RIS

TY - JOUR

T1 - V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element

AU - Roch, F A

AU - Hobi, R

AU - Berchtold, M W

AU - Kuenzle, C C

N1 - Keywords: Animals; Antibody Diversity; B-Lymphocytes; Base Composition; Base Sequence; Cell Line; Consensus Sequence; Enhancer Elements, Genetic; Gene Rearrangement, B-Lymphocyte; Humans; Immunoglobulin Heavy Chains; Immunoglobulin Joining Region; Immunoglobulin Variable Region; Introns; Mice; Molecular Sequence Data; Protein Sorting Signals; Recombinant Proteins; Recombination, Genetic; Regulatory Sequences, Nucleic Acid; Transcription, Genetic; Transfection

PY - 1997

Y1 - 1997

N2 - The immunoglobulin heavy chain intron enhancer (Emu) not only stimulates transcription but also V(D)J recombination of chromosomally integrated recombination substrates. We aimed at reproducing this effect in recombination competent cells by transient transfection of extrachromosomal substrates. These we prepared by interposing between the recombination signal sequences (RSS) of the plasmid pBlueRec various fragments, including Emu, possibly affecting V(D)J recombination. Our work shows that sequences inserted between RSS 23 and RSS 12, with distances from their proximal ends of 26 and 284 bp respectively, can markedly affect the frequency of V(D)J recombination. We report that the entire Emu, the Emu core as well as its flanking 5' and 3' matrix associated regions (5' and 3' MARs) upregulate V(D)J recombination while the downstream section of the 3' MAR of Emu does not. Also, prokaryotic sequences markedly suppress V(D)J recombination. This confirms previous results obtained with chromosomally integrated substrates, except for the finding that the full length 3' MAR of Emu stimulates V(D)J recombination in an episomal but not in a chromosomal context. The fact that other MARs do not share this activity suggests that the effect is no mediated through attachment of the recombination substrate to a nuclear matrix-associated recombination complex but through cis-activation. The presence of a 26 bp A-T-rich sequence motif in the 5' and 3' MARs of Emu and in all of the other upregulating fragments investigated, leads us to propose that the motif represents a novel recombinational enhancer element distinct from those constituting the Emu core.

AB - The immunoglobulin heavy chain intron enhancer (Emu) not only stimulates transcription but also V(D)J recombination of chromosomally integrated recombination substrates. We aimed at reproducing this effect in recombination competent cells by transient transfection of extrachromosomal substrates. These we prepared by interposing between the recombination signal sequences (RSS) of the plasmid pBlueRec various fragments, including Emu, possibly affecting V(D)J recombination. Our work shows that sequences inserted between RSS 23 and RSS 12, with distances from their proximal ends of 26 and 284 bp respectively, can markedly affect the frequency of V(D)J recombination. We report that the entire Emu, the Emu core as well as its flanking 5' and 3' matrix associated regions (5' and 3' MARs) upregulate V(D)J recombination while the downstream section of the 3' MAR of Emu does not. Also, prokaryotic sequences markedly suppress V(D)J recombination. This confirms previous results obtained with chromosomally integrated substrates, except for the finding that the full length 3' MAR of Emu stimulates V(D)J recombination in an episomal but not in a chromosomal context. The fact that other MARs do not share this activity suggests that the effect is no mediated through attachment of the recombination substrate to a nuclear matrix-associated recombination complex but through cis-activation. The presence of a 26 bp A-T-rich sequence motif in the 5' and 3' MARs of Emu and in all of the other upregulating fragments investigated, leads us to propose that the motif represents a novel recombinational enhancer element distinct from those constituting the Emu core.

M3 - Journal article

C2 - 9235545

VL - 25

SP - 2303

EP - 2310

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 12

ER -

ID: 11175580