Deciphering conjugative plasmid permissiveness in wastewater microbiomes
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Deciphering conjugative plasmid permissiveness in wastewater microbiomes. / Jacquiod, Samuel Jehan Auguste; Brejnrod, Asker Daniel; Milani, Stefan Morberg; Abu Al-Soud, Waleed; Sørensen, Søren Johannes; Riber, Leise.
In: Molecular Ecology, Vol. 26, No. 13, 07.2017, p. 3556-3571.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Deciphering conjugative plasmid permissiveness in wastewater microbiomes
AU - Jacquiod, Samuel Jehan Auguste
AU - Brejnrod, Asker Daniel
AU - Milani, Stefan Morberg
AU - Abu Al-Soud, Waleed
AU - Sørensen, Søren Johannes
AU - Riber, Leise
PY - 2017/7
Y1 - 2017/7
N2 - Wastewater treatment plants (WWTPs) are designed to robustly treat polluted water. They are characterized by ceaseless flows of organic, chemical and microbial matter, followed by treatment steps before environmental release. WWTPs are hotspots of horizontal gene transfer between bacteria via conjugative plasmids, leading to dissemination of potentially hazardous genetic material such as antimicrobial resistance genes (AMRGs). While current focus is on the threat of AMRGs spreading and their environmental maintenance, conjugative plasmid transfer dynamics within and between bacterial communities still remains largely uncharted. Furthermore, current in vitro methods used to assess conjugation in complex microbiomes do not include in situ behaviours of recipient cells, resulting in partial understanding of transfers. We investigated the in vitro conjugation capacities of WWTP microbiomes from inlet sewage and outlet treated water using the broad-host range IncP-1 conjugative plasmid, pKJK5. A thorough molecular approach coupling metagenomes to 16S rRNA DNA/cDNA amplicon sequencing was established to characterize microbiomes using the ecological concept of functional response groups. A broad diversity of recipient bacterial phyla for the plasmid was observed, especially in WWTP outlets. We also identified permissive bacteria potentially able to cross WWTPs and engage in conjugation before and after water treatment. Bacterial activity and lifestyle seem to influence conjugation extent, as treated water copiotrophs were the most represented strategist amongst transconjugants. Correlation analysis highlighted possible plasmid transmission routes into communities between the sewage to the environment, with identification of keystone members (e.g., Arcobacter) potentially involved in cross-border exchanges between distant Gram-positive and Gram-negative phyla.
AB - Wastewater treatment plants (WWTPs) are designed to robustly treat polluted water. They are characterized by ceaseless flows of organic, chemical and microbial matter, followed by treatment steps before environmental release. WWTPs are hotspots of horizontal gene transfer between bacteria via conjugative plasmids, leading to dissemination of potentially hazardous genetic material such as antimicrobial resistance genes (AMRGs). While current focus is on the threat of AMRGs spreading and their environmental maintenance, conjugative plasmid transfer dynamics within and between bacterial communities still remains largely uncharted. Furthermore, current in vitro methods used to assess conjugation in complex microbiomes do not include in situ behaviours of recipient cells, resulting in partial understanding of transfers. We investigated the in vitro conjugation capacities of WWTP microbiomes from inlet sewage and outlet treated water using the broad-host range IncP-1 conjugative plasmid, pKJK5. A thorough molecular approach coupling metagenomes to 16S rRNA DNA/cDNA amplicon sequencing was established to characterize microbiomes using the ecological concept of functional response groups. A broad diversity of recipient bacterial phyla for the plasmid was observed, especially in WWTP outlets. We also identified permissive bacteria potentially able to cross WWTPs and engage in conjugation before and after water treatment. Bacterial activity and lifestyle seem to influence conjugation extent, as treated water copiotrophs were the most represented strategist amongst transconjugants. Correlation analysis highlighted possible plasmid transmission routes into communities between the sewage to the environment, with identification of keystone members (e.g., Arcobacter) potentially involved in cross-border exchanges between distant Gram-positive and Gram-negative phyla.
KW - 16S rRNA
KW - cDNA
KW - conjugative plasmid
KW - metagenome
KW - wastewater
UR - http://www.scopus.com/inward/record.url?scp=85018447205&partnerID=8YFLogxK
U2 - 10.1111/mec.14138
DO - 10.1111/mec.14138
M3 - Journal article
C2 - 28390108
AN - SCOPUS:85018447205
VL - 26
SP - 3556
EP - 3571
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 13
ER -
ID: 181414952