Exploring the immediate and long-term impact on bacterial communities in soil amended with animal and urban organic waste fertilizers using pyrosequencing and screening for horizontal transfer of antibiotic resistance
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Exploring the immediate and long-term impact on bacterial communities in soil amended with animal and urban organic waste fertilizers using pyrosequencing and screening for horizontal transfer of antibiotic resistance. / Riber, Leise; Poulsen, Pernille Hasse Busk; Abu Al-Soud, Waleed; Hansen, Lea Benedicte Skov; Bergmark, Lasse; Brejnrod, Asker Daniel; Norman, Anders; Hansen, Lars H.; Magid, Jakob; Sørensen, Søren Johannes.
In: F E M S Microbiology Ecology, Vol. 90, No. 1, 2014, p. 206-224.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Exploring the immediate and long-term impact on bacterial communities in soil amended with animal and urban organic waste fertilizers using pyrosequencing and screening for horizontal transfer of antibiotic resistance
AU - Riber, Leise
AU - Poulsen, Pernille Hasse Busk
AU - Abu Al-Soud, Waleed
AU - Hansen, Lea Benedicte Skov
AU - Bergmark, Lasse
AU - Brejnrod, Asker Daniel
AU - Norman, Anders
AU - Hansen, Lars H.
AU - Magid, Jakob
AU - Sørensen, Søren Johannes
N1 - This article is protected by copyright. All rights reserved.
PY - 2014
Y1 - 2014
N2 - We investigated immediate and long-term effects on bacterial populations of soil amended with cattle manure, sewage sludge or municipal solid waste-compost in an on-going agricultural field trial. Soils were sampled in weeks 0, 3, 9 and 29 after fertilizer application. Pseudomonas isolates were enumerated, and the impact on soil-bacterial community structure was investigated using 16S-rRNA amplicon pyrosequencing. Bacterial community structure at phylum-level remained mostly unaffected. Actinobacteria, Proteobacteria and Chloroflexi were the most prevalent phyla significantly responding to sampling time. Seasonal changes seemed to prevail with decreasing bacterial richness in week 9 followed by a significant increase in week 29 (springtime). The Pseudomonas population richness seemed temporarily affected by fertilizer treatments, especially in sludge- and compost-amended soils. To explain these changes, prevalence of antibiotic- and mercury-resistant pseudomonads was investigated. Fertilizer amendment had a transient impact on the resistance profile of the soil community; abundance of resistant isolates decreased with time after fertilizer application, but persistent strains appeared multiresistant, also in unfertilized soil. Finally, the ability of a P. putida strain to take up resistance genes from indigenous soil bacteria by horizontal gene transfer was present only in week 0, indicating a temporary increase in prevalence of transferable antibiotic resistance genes. This article is protected by copyright. All rights reserved.
AB - We investigated immediate and long-term effects on bacterial populations of soil amended with cattle manure, sewage sludge or municipal solid waste-compost in an on-going agricultural field trial. Soils were sampled in weeks 0, 3, 9 and 29 after fertilizer application. Pseudomonas isolates were enumerated, and the impact on soil-bacterial community structure was investigated using 16S-rRNA amplicon pyrosequencing. Bacterial community structure at phylum-level remained mostly unaffected. Actinobacteria, Proteobacteria and Chloroflexi were the most prevalent phyla significantly responding to sampling time. Seasonal changes seemed to prevail with decreasing bacterial richness in week 9 followed by a significant increase in week 29 (springtime). The Pseudomonas population richness seemed temporarily affected by fertilizer treatments, especially in sludge- and compost-amended soils. To explain these changes, prevalence of antibiotic- and mercury-resistant pseudomonads was investigated. Fertilizer amendment had a transient impact on the resistance profile of the soil community; abundance of resistant isolates decreased with time after fertilizer application, but persistent strains appeared multiresistant, also in unfertilized soil. Finally, the ability of a P. putida strain to take up resistance genes from indigenous soil bacteria by horizontal gene transfer was present only in week 0, indicating a temporary increase in prevalence of transferable antibiotic resistance genes. This article is protected by copyright. All rights reserved.
U2 - 10.1111/1574-6941.12403
DO - 10.1111/1574-6941.12403
M3 - Journal article
C2 - 25087596
VL - 90
SP - 206
EP - 224
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 1
ER -
ID: 120837755