Expression of recombinant proteins: an introduction

Research output: Contribution to journalJournal articleResearchpeer-review

To better understand and characterize the function (or lack thereof) of proteins-wild-type vs mutants-it is advantageous to be able to express these proteins in a controlled, chosen setup. For downstream applications such as enzymatic assays, X-ray crystallography/nuclear magnetic resonance (NMR), raising antibodies, or characterization of physical interaction with other proteins, it may furthermore be desirable to obtain large quantities of pure protein.
Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)93-101
Number of pages8
Publication statusPublished - 2003
Externally publishedYes

Bibliographical note

Keywords: Animals; Biotechnology; Protein Engineering; Recombinant Proteins

ID: 11230768