Expression of recombinant proteins: an introduction
Research output: Contribution to journal › Journal article › Research › peer-review
To better understand and characterize the function (or lack thereof) of proteins-wild-type vs mutants-it is advantageous to be able to express these proteins in a controlled, chosen setup. For downstream applications such as enzymatic assays, X-ray crystallography/nuclear magnetic resonance (NMR), raising antibodies, or characterization of physical interaction with other proteins, it may furthermore be desirable to obtain large quantities of pure protein.
|Journal||Methods in Molecular Biology|
|Number of pages||8|
|Publication status||Published - 2003|
Keywords: Animals; Biotechnology; Protein Engineering; Recombinant Proteins