Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells

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Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells. / Dong, Guoyi; Shang, Zhouchun; Liu, Longqi; Liu, Chuanyu; Ge, Yuping; Wang, Quanlei; Wu, Liang; Chen, Fang; Li, Baolin; Liu, Xin; Xu, Xun; Yang, Huanming; Du, Yutao; Jiang, Hui.

I: Bioscience Reports, Bind 37, Nr. 2, BSR20170637, 2017.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Dong, G, Shang, Z, Liu, L, Liu, C, Ge, Y, Wang, Q, Wu, L, Chen, F, Li, B, Liu, X, Xu, X, Yang, H, Du, Y & Jiang, H 2017, 'Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells', Bioscience Reports, bind 37, nr. 2, BSR20170637. https://doi.org/10.1042/BSR20170637

APA

Dong, G., Shang, Z., Liu, L., Liu, C., Ge, Y., Wang, Q., Wu, L., Chen, F., Li, B., Liu, X., Xu, X., Yang, H., Du, Y., & Jiang, H. (2017). Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells. Bioscience Reports, 37(2), [BSR20170637]. https://doi.org/10.1042/BSR20170637

Vancouver

Dong G, Shang Z, Liu L, Liu C, Ge Y, Wang Q o.a. Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells. Bioscience Reports. 2017;37(2). BSR20170637. https://doi.org/10.1042/BSR20170637

Author

Dong, Guoyi ; Shang, Zhouchun ; Liu, Longqi ; Liu, Chuanyu ; Ge, Yuping ; Wang, Quanlei ; Wu, Liang ; Chen, Fang ; Li, Baolin ; Liu, Xin ; Xu, Xun ; Yang, Huanming ; Du, Yutao ; Jiang, Hui. / Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells. I: Bioscience Reports. 2017 ; Bind 37, Nr. 2.

Bibtex

@article{f5952c8b7a4d47c4b91ab7de10e1ab90,
title = "Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells",
abstract = "Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as Mvh, Dazl, Prdm14, Stella, Scp1, Scp3, Stra8 and Rec8. In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis in vitro.",
author = "Guoyi Dong and Zhouchun Shang and Longqi Liu and Chuanyu Liu and Yuping Ge and Quanlei Wang and Liang Wu and Fang Chen and Baolin Li and Xin Liu and Xun Xu and Huanming Yang and Yutao Du and Hui Jiang",
year = "2017",
doi = "10.1042/BSR20170637",
language = "English",
volume = "37",
journal = "Bioscience Reports",
issn = "0144-8463",
publisher = "Portland Press Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells

AU - Dong, Guoyi

AU - Shang, Zhouchun

AU - Liu, Longqi

AU - Liu, Chuanyu

AU - Ge, Yuping

AU - Wang, Quanlei

AU - Wu, Liang

AU - Chen, Fang

AU - Li, Baolin

AU - Liu, Xin

AU - Xu, Xun

AU - Yang, Huanming

AU - Du, Yutao

AU - Jiang, Hui

PY - 2017

Y1 - 2017

N2 - Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as Mvh, Dazl, Prdm14, Stella, Scp1, Scp3, Stra8 and Rec8. In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis in vitro.

AB - Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as Mvh, Dazl, Prdm14, Stella, Scp1, Scp3, Stra8 and Rec8. In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis in vitro.

U2 - 10.1042/BSR20170637

DO - 10.1042/BSR20170637

M3 - Journal article

C2 - 28314787

VL - 37

JO - Bioscience Reports

JF - Bioscience Reports

SN - 0144-8463

IS - 2

M1 - BSR20170637

ER -

ID: 182390281