Characterization of Divalent Cation Interactions with AASTY Nanodiscs

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Standard

Characterization of Divalent Cation Interactions with AASTY Nanodiscs. / Timcenko, Milena; Autzen, Anton A. A.; Autzen, Henriette E.

I: ACS Applied Polymer Materials, Bind 4, Nr. 2, 2022, s. 1071-1083.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Timcenko, M, Autzen, AAA & Autzen, HE 2022, 'Characterization of Divalent Cation Interactions with AASTY Nanodiscs', ACS Applied Polymer Materials, bind 4, nr. 2, s. 1071-1083. https://doi.org/10.1021/acsapm.1c01507

APA

Timcenko, M., Autzen, A. A. A., & Autzen, H. E. (2022). Characterization of Divalent Cation Interactions with AASTY Nanodiscs. ACS Applied Polymer Materials, 4(2), 1071-1083. https://doi.org/10.1021/acsapm.1c01507

Vancouver

Timcenko M, Autzen AAA, Autzen HE. Characterization of Divalent Cation Interactions with AASTY Nanodiscs. ACS Applied Polymer Materials. 2022;4(2):1071-1083. https://doi.org/10.1021/acsapm.1c01507

Author

Timcenko, Milena ; Autzen, Anton A. A. ; Autzen, Henriette E. / Characterization of Divalent Cation Interactions with AASTY Nanodiscs. I: ACS Applied Polymer Materials. 2022 ; Bind 4, Nr. 2. s. 1071-1083.

Bibtex

@article{2f5c438b25d44eda8cff1705cb12ac0f,
title = "Characterization of Divalent Cation Interactions with AASTY Nanodiscs",
abstract = "Amphiphilic copolymers show promise in extracting membrane proteins directly from lipid bilayers into {"}native nanodiscs{"}. However, many such copolymers are polyanionic and sensitive to divalent cations, limiting their applicability. We characterize the Ca2+ and Mg2+ sensitivity of poly(acrylic acid-co-styrene) (AASTY) copolymers with analytical UV and fluorescent size exclusion chromatography, enabling us to separate signals from nanodiscs, copolymers, and soluble aggregates. We find that divalent cations promote aggregation and precipitation of both free and lipid bound copolymers. We see that excess, free copolymer acts as a {"}cation sink{"}that protects nanodiscs from Ca2+ induced aggregation. Removal of the free copolymer through dialysis induces aggregation that can be mitigated by KCl. Finally, we find that the nanodisc size is dynamic and dependent on lipid concentration. Our results offer insight into nanodisc behavior and can help guide experimental design aimed at mitigating the shortcomings inherent in negatively charged nanodisc forming copolymers. ",
keywords = "AASTY copolymers, divalent cation tolerance, lipid biophysics, native nanodiscs, poly(acrylic acid- co-styrene), polymer chemistry",
author = "Milena Timcenko and Autzen, {Anton A. A.} and Autzen, {Henriette E.}",
note = "Publisher Copyright: {\textcopyright} ",
year = "2022",
doi = "10.1021/acsapm.1c01507",
language = "English",
volume = "4",
pages = "1071--1083",
journal = "ACS Applied Polymer Materials",
issn = "2637-6105",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Characterization of Divalent Cation Interactions with AASTY Nanodiscs

AU - Timcenko, Milena

AU - Autzen, Anton A. A.

AU - Autzen, Henriette E.

N1 - Publisher Copyright: ©

PY - 2022

Y1 - 2022

N2 - Amphiphilic copolymers show promise in extracting membrane proteins directly from lipid bilayers into "native nanodiscs". However, many such copolymers are polyanionic and sensitive to divalent cations, limiting their applicability. We characterize the Ca2+ and Mg2+ sensitivity of poly(acrylic acid-co-styrene) (AASTY) copolymers with analytical UV and fluorescent size exclusion chromatography, enabling us to separate signals from nanodiscs, copolymers, and soluble aggregates. We find that divalent cations promote aggregation and precipitation of both free and lipid bound copolymers. We see that excess, free copolymer acts as a "cation sink"that protects nanodiscs from Ca2+ induced aggregation. Removal of the free copolymer through dialysis induces aggregation that can be mitigated by KCl. Finally, we find that the nanodisc size is dynamic and dependent on lipid concentration. Our results offer insight into nanodisc behavior and can help guide experimental design aimed at mitigating the shortcomings inherent in negatively charged nanodisc forming copolymers.

AB - Amphiphilic copolymers show promise in extracting membrane proteins directly from lipid bilayers into "native nanodiscs". However, many such copolymers are polyanionic and sensitive to divalent cations, limiting their applicability. We characterize the Ca2+ and Mg2+ sensitivity of poly(acrylic acid-co-styrene) (AASTY) copolymers with analytical UV and fluorescent size exclusion chromatography, enabling us to separate signals from nanodiscs, copolymers, and soluble aggregates. We find that divalent cations promote aggregation and precipitation of both free and lipid bound copolymers. We see that excess, free copolymer acts as a "cation sink"that protects nanodiscs from Ca2+ induced aggregation. Removal of the free copolymer through dialysis induces aggregation that can be mitigated by KCl. Finally, we find that the nanodisc size is dynamic and dependent on lipid concentration. Our results offer insight into nanodisc behavior and can help guide experimental design aimed at mitigating the shortcomings inherent in negatively charged nanodisc forming copolymers.

KW - AASTY copolymers

KW - divalent cation tolerance

KW - lipid biophysics

KW - native nanodiscs

KW - poly(acrylic acid- co-styrene)

KW - polymer chemistry

U2 - 10.1021/acsapm.1c01507

DO - 10.1021/acsapm.1c01507

M3 - Journal article

AN - SCOPUS:85124667920

VL - 4

SP - 1071

EP - 1083

JO - ACS Applied Polymer Materials

JF - ACS Applied Polymer Materials

SN - 2637-6105

IS - 2

ER -

ID: 298631992