Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control
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Intensive aquaculture conditions expose fish to bacterial infections, leading to significant financial losses, extensive antibiotic use and risk of antibiotic resistance in target bacteria. Flavobacterium columnare causes columnaris disease in aquaculture worldwide. To develop a bacteriophage-based control of columnaris disease, we isolated and characterized 126 F. columnare strains and 63 phages against F. columnare from Finland and Sweden in 2017. Bacterial isolates were virulent on rainbow trout (Oncorhynchus mykiss) and fell into four previously described genetic groups A, C, E and G, with genetic groups C and E being the most virulent. Phage host range studied against a collection of 227 bacterial isolates (from 2013 to 2017) demonstrated modular infection patterns based on host genetic group. Phages infected contemporary and previously isolated bacterial hosts, but bacteria isolated most recently were generally resistant to previously isolated phages. Despite large differences in geographical origin, isolation year or host range of the phages, whole-genome sequencing of 56 phages showed high level of genetic similarity to previously isolated F. columnare phages (Ficleduovirus, Myoviridae). Altogether, this phage collection demonstrates a potential for use in phage therapy.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Environmental Microbiology |
| Vol/bind | 24 |
| Udgave nummer | 5 |
| Sider (fra-til) | 2404-2420 |
| Antal sider | 17 |
| ISSN | 1462-2912 |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This work resulted from the BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Academy of Finland and Innovation Fund Denmark. We also acknowledge funding from the Academy of Finland (grants #314939 and #321985), the Jane and Aatos Erkko Foundation and the Kone Foundation. The authors wish to thank Dr. Päivi Rintamäki (University of Oulu), Eva Jansson (National Veterinary Institute of Sweden), Mr Yrjö Lankinen and Natural research Institute Finland for donating water samples and bacterial isolates, and Ms. Hannah Kempf, Dr. Roghaieh Ashrafi and special laboratory technician Mr. Petri Papponen for technical assistance. We also thank Jean‐Francois Bernardet (INRA, France), Mark McBride (UWM, USA) and Attila Karsi (Mississippi State University, USA) for providing bacterial strains for phage host range testing. L.‐R.S., G.M.F.A., E.L., and the University of Jyväskylä are responsible for a patent application covering the commercial use of purified mucin for production, quantification and isolation of bacteriophages. It is titled ‘Improved methods and culture media for production, quantification and isolation of bacteriophages’ and was filed with the Finnish Patent and Registration Office under patent no. FI20185086 (PCT/FI2019/050073) on 31 January 2018.
Publisher Copyright:
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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