A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments. / Norman, Anders; Hansen, Lars H.; Sørensen, Søren Johannes.

In: Mutation Research - Reviews, Vol. 603, No. 2, 2006, p. 164-172.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Norman, A, Hansen, LH & Sørensen, SJ 2006, 'A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments', Mutation Research - Reviews, vol. 603, no. 2, pp. 164-172. https://doi.org/10.1016/j.mrgentox.2005.11.008

APA

Norman, A., Hansen, L. H., & Sørensen, S. J. (2006). A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments. Mutation Research - Reviews, 603(2), 164-172. https://doi.org/10.1016/j.mrgentox.2005.11.008

Vancouver

Norman A, Hansen LH, Sørensen SJ. A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments. Mutation Research - Reviews. 2006;603(2):164-172. https://doi.org/10.1016/j.mrgentox.2005.11.008

Author

Norman, Anders ; Hansen, Lars H. ; Sørensen, Søren Johannes. / A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments. In: Mutation Research - Reviews. 2006 ; Vol. 603, No. 2. pp. 164-172.

Bibtex

@article{31371840ad7711dcbee902004c4f4f50,
title = "A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments",
abstract = "Whole-cell biosensors have become popular tools for detection of ecotoxic compounds in environmental samples. We have developed an assay optimized for flow cytometry with detection of genotoxic compounds in mind. The assay features extended pre-incubation and a cell density of only 106-107 cells/mL, and proved far more sensitive than a previously published assay using the same biosensor strain. By applying the SOS-green fluorescent protein (GFP) whole-cell biosensor directly to soil microcosms we were also able to evaluate both the applicability and sensitivity of a biosensor based on SOS-induction in whole soil samples. Soil microcosms were spiked with a dilution-series of crude broth extract from the mitomycin C-producing streptomycete Streptomyces caespitosus. Biosensors extracted from these microcosms after 1 day of incubation at 30 °C were easily distinguished from extracts of non-contaminated soil particles when using flow cytometry, and induction of the biosensor by mitomycin C was detectable at concentrations as low as 2.5 ng/g of soil.",
author = "Anders Norman and Hansen, {Lars H.} and S{\o}rensen, {S{\o}ren Johannes}",
note = "Keywords: SOS-response; Green fluorescent protein (GFP); Whole-cell biosensor; Flow cytometry; Genotoxicity; Mitomycin C; Soil microcosms",
year = "2006",
doi = "10.1016/j.mrgentox.2005.11.008",
language = "English",
volume = "603",
pages = "164--172",
journal = "Mutation Research - Reviews",
issn = "1383-5742",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments

AU - Norman, Anders

AU - Hansen, Lars H.

AU - Sørensen, Søren Johannes

N1 - Keywords: SOS-response; Green fluorescent protein (GFP); Whole-cell biosensor; Flow cytometry; Genotoxicity; Mitomycin C; Soil microcosms

PY - 2006

Y1 - 2006

N2 - Whole-cell biosensors have become popular tools for detection of ecotoxic compounds in environmental samples. We have developed an assay optimized for flow cytometry with detection of genotoxic compounds in mind. The assay features extended pre-incubation and a cell density of only 106-107 cells/mL, and proved far more sensitive than a previously published assay using the same biosensor strain. By applying the SOS-green fluorescent protein (GFP) whole-cell biosensor directly to soil microcosms we were also able to evaluate both the applicability and sensitivity of a biosensor based on SOS-induction in whole soil samples. Soil microcosms were spiked with a dilution-series of crude broth extract from the mitomycin C-producing streptomycete Streptomyces caespitosus. Biosensors extracted from these microcosms after 1 day of incubation at 30 °C were easily distinguished from extracts of non-contaminated soil particles when using flow cytometry, and induction of the biosensor by mitomycin C was detectable at concentrations as low as 2.5 ng/g of soil.

AB - Whole-cell biosensors have become popular tools for detection of ecotoxic compounds in environmental samples. We have developed an assay optimized for flow cytometry with detection of genotoxic compounds in mind. The assay features extended pre-incubation and a cell density of only 106-107 cells/mL, and proved far more sensitive than a previously published assay using the same biosensor strain. By applying the SOS-green fluorescent protein (GFP) whole-cell biosensor directly to soil microcosms we were also able to evaluate both the applicability and sensitivity of a biosensor based on SOS-induction in whole soil samples. Soil microcosms were spiked with a dilution-series of crude broth extract from the mitomycin C-producing streptomycete Streptomyces caespitosus. Biosensors extracted from these microcosms after 1 day of incubation at 30 °C were easily distinguished from extracts of non-contaminated soil particles when using flow cytometry, and induction of the biosensor by mitomycin C was detectable at concentrations as low as 2.5 ng/g of soil.

U2 - 10.1016/j.mrgentox.2005.11.008

DO - 10.1016/j.mrgentox.2005.11.008

M3 - Journal article

VL - 603

SP - 164

EP - 172

JO - Mutation Research - Reviews

JF - Mutation Research - Reviews

SN - 1383-5742

IS - 2

ER -

ID: 1913435