A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer: Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer : Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer. / Glud, Ronnie N.; Rysgaard, Søren; Kühl, Michael.

In: Aquatic Microbial Ecology, Vol. 27, No. 3, 16.04.2002, p. 301-311.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Glud, RN, Rysgaard, S & Kühl, M 2002, 'A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer: Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer', Aquatic Microbial Ecology, vol. 27, no. 3, pp. 301-311. https://doi.org/10.3354/ame027301

APA

Glud, R. N., Rysgaard, S., & Kühl, M. (2002). A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer: Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer. Aquatic Microbial Ecology, 27(3), 301-311. https://doi.org/10.3354/ame027301

Vancouver

Glud RN, Rysgaard S, Kühl M. A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer: Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer. Aquatic Microbial Ecology. 2002 Apr 16;27(3):301-311. https://doi.org/10.3354/ame027301

Author

Glud, Ronnie N. ; Rysgaard, Søren ; Kühl, Michael. / A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer : Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer. In: Aquatic Microbial Ecology. 2002 ; Vol. 27, No. 3. pp. 301-311.

Bibtex

@article{ae01e26074c511dbbee902004c4f4f50,
title = "A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer: Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer",
abstract = "The present study compared O2 exchange measurements, fluorometry and 14c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O2, while the melting water released during thawing is undersaturated with O2. This results in a highly variable O2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement of O2 concentrations in water below sea ice. Accounting for the O2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O2 exchange rates and 14C incubation, co-varied as a function of biomass and irradiance. The ratio between the net O2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 ± 0.48. Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F0) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities. A similar observation was made for the correlation between measurements and the concentration of chlorophyll a.",
keywords = "Fluorometer, Microelectrodes, Oxygen, Primary production, Pulse amplitude modulated fluorometry, Sea-ice algae",
author = "Glud, {Ronnie N.} and S{\o}ren Rysgaard and Michael K{\"u}hl",
year = "2002",
month = apr,
day = "16",
doi = "10.3354/ame027301",
language = "English",
volume = "27",
pages = "301--311",
journal = "Aquatic Microbial Ecology",
issn = "0948-3055",
publisher = "Inter research",
number = "3",

}

RIS

TY - JOUR

T1 - A laboratory study on O2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer

T2 - Quantification by microsensors, O2 exchange rates, 14C incubations and a PAM fluorometer

AU - Glud, Ronnie N.

AU - Rysgaard, Søren

AU - Kühl, Michael

PY - 2002/4/16

Y1 - 2002/4/16

N2 - The present study compared O2 exchange measurements, fluorometry and 14c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O2, while the melting water released during thawing is undersaturated with O2. This results in a highly variable O2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement of O2 concentrations in water below sea ice. Accounting for the O2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O2 exchange rates and 14C incubation, co-varied as a function of biomass and irradiance. The ratio between the net O2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 ± 0.48. Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F0) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities. A similar observation was made for the correlation between measurements and the concentration of chlorophyll a.

AB - The present study compared O2 exchange measurements, fluorometry and 14c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O2, while the melting water released during thawing is undersaturated with O2. This results in a highly variable O2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement of O2 concentrations in water below sea ice. Accounting for the O2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O2 exchange rates and 14C incubation, co-varied as a function of biomass and irradiance. The ratio between the net O2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 ± 0.48. Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F0) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities. A similar observation was made for the correlation between measurements and the concentration of chlorophyll a.

KW - Fluorometer

KW - Microelectrodes

KW - Oxygen

KW - Primary production

KW - Pulse amplitude modulated fluorometry

KW - Sea-ice algae

UR - http://www.scopus.com/inward/record.url?scp=0037117811&partnerID=8YFLogxK

U2 - 10.3354/ame027301

DO - 10.3354/ame027301

M3 - Journal article

AN - SCOPUS:0037117811

VL - 27

SP - 301

EP - 311

JO - Aquatic Microbial Ecology

JF - Aquatic Microbial Ecology

SN - 0948-3055

IS - 3

ER -

ID: 136948