A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples. / Holst, Gerhard; Kohls, Oliver; Klimant, Ingo; König, Bettina; Kühl, Michael; Richter, Thomas.

In: Sensors and Actuators, B: Chemical, Vol. 51, No. 1-3, 1998, p. 163-170.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Holst, G, Kohls, O, Klimant, I, König, B, Kühl, M & Richter, T 1998, 'A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples', Sensors and Actuators, B: Chemical, vol. 51, no. 1-3, pp. 163-170. https://doi.org/10.1016/S0925-4005(98)00232-9

APA

Holst, G., Kohls, O., Klimant, I., König, B., Kühl, M., & Richter, T. (1998). A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples. Sensors and Actuators, B: Chemical, 51(1-3), 163-170. https://doi.org/10.1016/S0925-4005(98)00232-9

Vancouver

Holst G, Kohls O, Klimant I, König B, Kühl M, Richter T. A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples. Sensors and Actuators, B: Chemical. 1998;51(1-3):163-170. https://doi.org/10.1016/S0925-4005(98)00232-9

Author

Holst, Gerhard ; Kohls, Oliver ; Klimant, Ingo ; König, Bettina ; Kühl, Michael ; Richter, Thomas. / A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples. In: Sensors and Actuators, B: Chemical. 1998 ; Vol. 51, No. 1-3. pp. 163-170.

Bibtex

@article{cf64184b919c4d149b6971e96bad387b,
title = "A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples",
abstract = "We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.",
keywords = "Fluorescence lifetime imaging, Luminescence lifetime imaging, Oxygen optode, Planar optode",
author = "Gerhard Holst and Oliver Kohls and Ingo Klimant and Bettina K{\"o}nig and Michael K{\"u}hl and Thomas Richter",
year = "1998",
doi = "10.1016/S0925-4005(98)00232-9",
language = "English",
volume = "51",
pages = "163--170",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1-3",

}

RIS

TY - JOUR

T1 - A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

AU - Holst, Gerhard

AU - Kohls, Oliver

AU - Klimant, Ingo

AU - König, Bettina

AU - Kühl, Michael

AU - Richter, Thomas

PY - 1998

Y1 - 1998

N2 - We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.

AB - We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.

KW - Fluorescence lifetime imaging

KW - Luminescence lifetime imaging

KW - Oxygen optode

KW - Planar optode

U2 - 10.1016/S0925-4005(98)00232-9

DO - 10.1016/S0925-4005(98)00232-9

M3 - Journal article

AN - SCOPUS:0032130963

VL - 51

SP - 163

EP - 170

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

IS - 1-3

ER -

ID: 201682581