A strand specific high resolution normalization method for chip-sequencing data employing multiple experimental control measurements.

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  • A strand...

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  • Stefan Enroth
  • Claes Andersson
  • Andersson, Robin
  • Claes Wadelius
  • Mats Gustafsson
  • Jan Komorowski
High-throughput sequencing is becoming the standard tool for investigating protein-DNA interactions or epigenetic modifications. However, the data generated will always contain noise due to e.g. repetitive regions or non-specific antibody interactions. The noise will appear in the form of a background distribution of reads that must be taken into account in the downstream analysis, for example when detecting enriched regions (peak-calling). Several reported peak-callers can take experimental measurements of background tag distribution into account when analysing a data set. Unfortunately, the background is only used to adjust peak calling and not as a pre-processing step that aims at discerning the signal from the background noise. A normalization procedure that extracts the signal of interest would be of universal use when investigating genomic patterns.
Original languageEnglish
JournalAlgorithms for Molecular Biology
Issue number1
Pages (from-to)2
Number of pages10
Publication statusPublished - 2012

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