A YTHDF–PABP interaction is required for m6A-mediated organogenesis in plants
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
A YTHDF–PABP interaction is required for m6A-mediated organogenesis in plants. / Due Tankmar, Mathias; Reichel, Marlene; Arribas-Hernández, Laura; Brodersen, Peter.
In: EMBO Reports, Vol. 24, No. 12, e57741, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - A YTHDF–PABP interaction is required for m6A-mediated organogenesis in plants
AU - Due Tankmar, Mathias
AU - Reichel, Marlene
AU - Arribas-Hernández, Laura
AU - Brodersen, Peter
N1 - Publisher Copyright: © 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
PY - 2023
Y1 - 2023
N2 - N6-methyladenosine (m6A) in mRNA is key to eukaryotic gene regulation. Many m6A functions involve RNA-binding proteins that recognize m6A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction with protein partners, but whose precise biochemical functions remain largely unknown. The Arabidopsis thaliana YTH domain proteins ECT2, ECT3, and ECT4 accelerate organogenesis through stimulation of cell division in organ primordia. Here, we use ECT2 to reveal molecular underpinnings of this function. We show that stimulation of leaf formation requires the long N-terminal IDR, and we identify two short IDR elements required for ECT2-mediated organogenesis. Of these two, a 19-amino acid region containing a tyrosine-rich motif conserved in both plant and metazoan YTHDF proteins is necessary for binding to the major cytoplasmic poly(A)-binding proteins PAB2, PAB4, and PAB8. Remarkably, overexpression of PAB4 in leaf primordia partially rescues the delayed leaf formation in ect2 ect3 ect4 mutants, suggesting that the ECT2-PAB2/4/8 interaction on target mRNAs of organogenesis-related genes may overcome limiting PAB concentrations in primordial cells.
AB - N6-methyladenosine (m6A) in mRNA is key to eukaryotic gene regulation. Many m6A functions involve RNA-binding proteins that recognize m6A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction with protein partners, but whose precise biochemical functions remain largely unknown. The Arabidopsis thaliana YTH domain proteins ECT2, ECT3, and ECT4 accelerate organogenesis through stimulation of cell division in organ primordia. Here, we use ECT2 to reveal molecular underpinnings of this function. We show that stimulation of leaf formation requires the long N-terminal IDR, and we identify two short IDR elements required for ECT2-mediated organogenesis. Of these two, a 19-amino acid region containing a tyrosine-rich motif conserved in both plant and metazoan YTHDF proteins is necessary for binding to the major cytoplasmic poly(A)-binding proteins PAB2, PAB4, and PAB8. Remarkably, overexpression of PAB4 in leaf primordia partially rescues the delayed leaf formation in ect2 ect3 ect4 mutants, suggesting that the ECT2-PAB2/4/8 interaction on target mRNAs of organogenesis-related genes may overcome limiting PAB concentrations in primordial cells.
KW - Arabidopsis
KW - ECT2
KW - IDR
KW - mA
KW - PABP
U2 - 10.15252/embr.202357741
DO - 10.15252/embr.202357741
M3 - Journal article
C2 - 38009565
AN - SCOPUS:85177807449
VL - 24
JO - E M B O Reports
JF - E M B O Reports
SN - 1469-221X
IS - 12
M1 - e57741
ER -
ID: 375297042