Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments. / Puentes-Téllez, Pilar; Hansen, Martin Asser; Sørensen, Søren; van Elsas, Jan.

In: Applied and Environmental Microbiology, Vol. 79, No. 3, 2013, p. 1008-1017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Puentes-Téllez, P, Hansen, MA, Sørensen, S & van Elsas, J 2013, 'Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments', Applied and Environmental Microbiology, vol. 79, no. 3, pp. 1008-1017. https://doi.org/10.1128/AEM.02920-12

APA

Puentes-Téllez, P., Hansen, M. A., Sørensen, S., & van Elsas, J. (2013). Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments. Applied and Environmental Microbiology, 79(3), 1008-1017. https://doi.org/10.1128/AEM.02920-12

Vancouver

Puentes-Téllez P, Hansen MA, Sørensen S, van Elsas J. Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments. Applied and Environmental Microbiology. 2013;79(3):1008-1017. https://doi.org/10.1128/AEM.02920-12

Author

Puentes-Téllez, Pilar ; Hansen, Martin Asser ; Sørensen, Søren ; van Elsas, Jan. / Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments. In: Applied and Environmental Microbiology. 2013 ; Vol. 79, No. 3. pp. 1008-1017.

Bibtex

@article{35d395a18c144d83a3c06f4121d9ffbc,
title = "Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments",
abstract = "In a study aiming to assess bacterial evolution in complex growth media, we evaluated the long-term adaptive response of Escherichia coli MC1000 in Luria-Bertani (LB) medium. Seven parallel populations were founded and followed over 150 days in sequential batch cultures under three different oxygen conditions (defined environments), and 19 evolved forms were isolated. The emergence of forms with enhanced fitness was evident in competition experiments of all evolved forms versus the ancestral strain. The evolved forms were then subjected to phenotypic and genomic analyses relative to the ancestor. Profound changes were found in their phenotypes as well as whole-genome sequences. Interestingly, considerable heterogeneity was found at the intrapopulational level. However, consistently occurring parallel adaptive responses were found across all populations. The evolved forms all contained a mutation in galR, a repressor of the galactose operon. Concomitantly, the new forms revealed enhanced growth on galactose as well as galactose-containing disaccharides. This response was likely driven by the LB medium.",
author = "Pilar Puentes-T{\'e}llez and Hansen, {Martin Asser} and S{\o}ren S{\o}rensen and {van Elsas}, Jan",
year = "2013",
doi = "10.1128/AEM.02920-12",
language = "English",
volume = "79",
pages = "1008--1017",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Adaptation and heterogeneity of Escherichia coli MC1000 growing in complex environments

AU - Puentes-Téllez, Pilar

AU - Hansen, Martin Asser

AU - Sørensen, Søren

AU - van Elsas, Jan

PY - 2013

Y1 - 2013

N2 - In a study aiming to assess bacterial evolution in complex growth media, we evaluated the long-term adaptive response of Escherichia coli MC1000 in Luria-Bertani (LB) medium. Seven parallel populations were founded and followed over 150 days in sequential batch cultures under three different oxygen conditions (defined environments), and 19 evolved forms were isolated. The emergence of forms with enhanced fitness was evident in competition experiments of all evolved forms versus the ancestral strain. The evolved forms were then subjected to phenotypic and genomic analyses relative to the ancestor. Profound changes were found in their phenotypes as well as whole-genome sequences. Interestingly, considerable heterogeneity was found at the intrapopulational level. However, consistently occurring parallel adaptive responses were found across all populations. The evolved forms all contained a mutation in galR, a repressor of the galactose operon. Concomitantly, the new forms revealed enhanced growth on galactose as well as galactose-containing disaccharides. This response was likely driven by the LB medium.

AB - In a study aiming to assess bacterial evolution in complex growth media, we evaluated the long-term adaptive response of Escherichia coli MC1000 in Luria-Bertani (LB) medium. Seven parallel populations were founded and followed over 150 days in sequential batch cultures under three different oxygen conditions (defined environments), and 19 evolved forms were isolated. The emergence of forms with enhanced fitness was evident in competition experiments of all evolved forms versus the ancestral strain. The evolved forms were then subjected to phenotypic and genomic analyses relative to the ancestor. Profound changes were found in their phenotypes as well as whole-genome sequences. Interestingly, considerable heterogeneity was found at the intrapopulational level. However, consistently occurring parallel adaptive responses were found across all populations. The evolved forms all contained a mutation in galR, a repressor of the galactose operon. Concomitantly, the new forms revealed enhanced growth on galactose as well as galactose-containing disaccharides. This response was likely driven by the LB medium.

U2 - 10.1128/AEM.02920-12

DO - 10.1128/AEM.02920-12

M3 - Journal article

C2 - 23204426

VL - 79

SP - 1008

EP - 1017

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 3

ER -

ID: 44755748