All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant. / Bahl, Martin Iain; Burmølle, Mette; Meisner, Annelein; Hansen, Lars H.; Sørensen, Søren Johannes.

In: Plasmid, Vol. 62, No. 2, 2009, p. 134-139.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bahl, MI, Burmølle, M, Meisner, A, Hansen, LH & Sørensen, SJ 2009, 'All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant', Plasmid, vol. 62, no. 2, pp. 134-139. https://doi.org/10.1016/j.plasmid.2009.05.004

APA

Bahl, M. I., Burmølle, M., Meisner, A., Hansen, L. H., & Sørensen, S. J. (2009). All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant. Plasmid, 62(2), 134-139. https://doi.org/10.1016/j.plasmid.2009.05.004

Vancouver

Bahl MI, Burmølle M, Meisner A, Hansen LH, Sørensen SJ. All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant. Plasmid. 2009;62(2):134-139. https://doi.org/10.1016/j.plasmid.2009.05.004

Author

Bahl, Martin Iain ; Burmølle, Mette ; Meisner, Annelein ; Hansen, Lars H. ; Sørensen, Søren Johannes. / All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant. In: Plasmid. 2009 ; Vol. 62, No. 2. pp. 134-139.

Bibtex

@article{181a8990eb1011deba73000ea68e967b,
title = "All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant",
abstract = "The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, a, {\ss}, ¿ and d, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the e subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes.",
author = "Bahl, {Martin Iain} and Mette Burm{\o}lle and Annelein Meisner and Hansen, {Lars H.} and S{\o}rensen, {S{\o}ren Johannes}",
note = "Keywords: IncP-1; Plasmid; PCR; Phylogeny; Wastewater",
year = "2009",
doi = "10.1016/j.plasmid.2009.05.004",
language = "English",
volume = "62",
pages = "134--139",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - All IncP-1 plasmid subgroups, including the novel e subgroup, are prevalent in the influent of a Danish wastewater treatment plant

AU - Bahl, Martin Iain

AU - Burmølle, Mette

AU - Meisner, Annelein

AU - Hansen, Lars H.

AU - Sørensen, Søren Johannes

N1 - Keywords: IncP-1; Plasmid; PCR; Phylogeny; Wastewater

PY - 2009

Y1 - 2009

N2 - The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, a, ß, ¿ and d, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the e subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes.

AB - The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, a, ß, ¿ and d, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the e subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes.

U2 - 10.1016/j.plasmid.2009.05.004

DO - 10.1016/j.plasmid.2009.05.004

M3 - Journal article

C2 - 19501117

VL - 62

SP - 134

EP - 139

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 2

ER -

ID: 16331012