Ancient conservation of trinucleotide microsatellite loci in polistine wasps
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Ancient conservation of trinucleotide microsatellite loci in polistine wasps. / Ezenwa, V O; Peters, J M; Zhu, Y; Arévalo, E; Hastings, M D; Seppä, P; Pedersen, Jes Søe; Zacchi, F; Queller, D C; Strassmann, J E.
In: Molecular Phylogenetics and Evolution, Vol. 10, No. 2, 10.1998, p. 168-77.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ancient conservation of trinucleotide microsatellite loci in polistine wasps
AU - Ezenwa, V O
AU - Peters, J M
AU - Zhu, Y
AU - Arévalo, E
AU - Hastings, M D
AU - Seppä, P
AU - Pedersen, Jes Søe
AU - Zacchi, F
AU - Queller, D C
AU - Strassmann, J E
N1 - Copyright 1998 Academic Press.
PY - 1998/10
Y1 - 1998/10
N2 - Microsatellites have proven to be very useful genetic markers for studies of kinship, parentage, and gene mapping. If microsatellites are conserved among species, then those developed for one species can be used on related species, which would save the time and effort of developing new loci. We evaluated conservation of 27 trinucleotide loci that were derived from 2 species of Polistes wasps in cross-species applications on 27 species chosen from the major lineages of the Vespidae, which diverged as much as 144 million years ago. We further investigated cross-species polymorphism levels for 18 of the loci. There was a clear relationship between cladistic distance and both conservation of the priming sites and heterozygosity. However the loci derived from P. bellicosus were much more widely conserved and polymorphic than were those derived from P. annularis. The disparity in cross-species utility between these sets of loci means that caution should be used in generalizing from conservation rates derived from single species. We found no relationship between locus conservation or heterozygosity and GC content of flanks, repeat motif, repeat length, or heterozygosity in the original species, which suggests that generalizations from other studies reporting such patterns are premature.
AB - Microsatellites have proven to be very useful genetic markers for studies of kinship, parentage, and gene mapping. If microsatellites are conserved among species, then those developed for one species can be used on related species, which would save the time and effort of developing new loci. We evaluated conservation of 27 trinucleotide loci that were derived from 2 species of Polistes wasps in cross-species applications on 27 species chosen from the major lineages of the Vespidae, which diverged as much as 144 million years ago. We further investigated cross-species polymorphism levels for 18 of the loci. There was a clear relationship between cladistic distance and both conservation of the priming sites and heterozygosity. However the loci derived from P. bellicosus were much more widely conserved and polymorphic than were those derived from P. annularis. The disparity in cross-species utility between these sets of loci means that caution should be used in generalizing from conservation rates derived from single species. We found no relationship between locus conservation or heterozygosity and GC content of flanks, repeat motif, repeat length, or heterozygosity in the original species, which suggests that generalizations from other studies reporting such patterns are premature.
KW - Animals
KW - Cold Temperature
KW - DNA
KW - Polymerase Chain Reaction
KW - Polymorphism, Genetic
KW - Species Specificity
KW - Trinucleotide Repeats
KW - Wasps
U2 - 10.1006/mpev.1998.0528
DO - 10.1006/mpev.1998.0528
M3 - Journal article
C2 - 9878228
VL - 10
SP - 168
EP - 177
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
IS - 2
ER -
ID: 33828120