Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
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Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells. / Rolver, Michala G.; Elingaard-Larsen, Line O.; Pedersen, Stine F.
In: Journal of Visualized Experiments, Vol. 2019, No. 148, e59714, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
AU - Rolver, Michala G.
AU - Elingaard-Larsen, Line O.
AU - Pedersen, Stine F.
PY - 2019
Y1 - 2019
N2 - Three-dimensional spheroids of cancer cells are important tools for both cancer drug screens and for gaining mechanistic insight into cancer cell biology. The power of this preparation lies in its ability to mimic many aspects of the in vivo conditions of tumors while being fast, cheap, and versatile enough to allow relatively high-throughput screening. The spheroid culture conditions can recapitulate the physico-chemical gradients in a tumor, including the increasing extracellular acidity, increased lactate, and decreasing glucose and oxygen availability, from the spheroid periphery to its core. Also, the mechanical properties and cell-cell interactions of in vivo tumors are in part mimicked by this model. The specific properties and consequently the optimal growth conditions, of 3D spheroids, differ widely between different types of cancer cells. Furthermore, the assessment of cell viability and death in 3D spheroids requires methods that differ in part from those employed for 2D cultures. Here we describe several protocols for preparing 3D spheroids of cancer cells, and for using such cultures to assess cell viability and death in the context of evaluating the efficacy of anticancer drugs.
AB - Three-dimensional spheroids of cancer cells are important tools for both cancer drug screens and for gaining mechanistic insight into cancer cell biology. The power of this preparation lies in its ability to mimic many aspects of the in vivo conditions of tumors while being fast, cheap, and versatile enough to allow relatively high-throughput screening. The spheroid culture conditions can recapitulate the physico-chemical gradients in a tumor, including the increasing extracellular acidity, increased lactate, and decreasing glucose and oxygen availability, from the spheroid periphery to its core. Also, the mechanical properties and cell-cell interactions of in vivo tumors are in part mimicked by this model. The specific properties and consequently the optimal growth conditions, of 3D spheroids, differ widely between different types of cancer cells. Furthermore, the assessment of cell viability and death in 3D spheroids requires methods that differ in part from those employed for 2D cultures. Here we describe several protocols for preparing 3D spheroids of cancer cells, and for using such cultures to assess cell viability and death in the context of evaluating the efficacy of anticancer drugs.
KW - 3D cell culture
KW - Anti-cancer therapy
KW - Breast cancer
KW - Cancer Research
KW - Cell viability
KW - Chemotherapy
KW - Drug treatment
KW - Hanging drop
KW - Immunohistochemistry
KW - Issue 148
KW - Pancreatic cancer
KW - Propidium iodide
KW - Reconstituted basement membrane
KW - Spheroids
U2 - 10.3791/59714
DO - 10.3791/59714
M3 - Journal article
C2 - 31259899
AN - SCOPUS:85068964018
VL - 2019
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 148
M1 - e59714
ER -
ID: 234024117