Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria. / Lima, Stella T.; Fallon, Timothy R.; Cordoza, Jennifer L.; Chekan, Jonathan R.; Delbaje, Endrews; Hopiavuori, Austin R.; Alvarenga, Danillo O.; Wood, Steffaney M.; Luhavaya, Hanna; Baumgartner, Jackson T.; Dörr, Felipe A.; Etchegaray, Augusto; Pinto, Ernani; McKinnie, Shaun M. K.; Fiore, Marli F.; Moore, Bradley S.

In: Journal of the American Chemical Society, Vol. 144, No. 21, 2022, p. 9372-9379.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lima, ST, Fallon, TR, Cordoza, JL, Chekan, JR, Delbaje, E, Hopiavuori, AR, Alvarenga, DO, Wood, SM, Luhavaya, H, Baumgartner, JT, Dörr, FA, Etchegaray, A, Pinto, E, McKinnie, SMK, Fiore, MF & Moore, BS 2022, 'Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria', Journal of the American Chemical Society, vol. 144, no. 21, pp. 9372-9379. https://doi.org/10.1021/jacs.2c01424

APA

Lima, S. T., Fallon, T. R., Cordoza, J. L., Chekan, J. R., Delbaje, E., Hopiavuori, A. R., Alvarenga, D. O., Wood, S. M., Luhavaya, H., Baumgartner, J. T., Dörr, F. A., Etchegaray, A., Pinto, E., McKinnie, S. M. K., Fiore, M. F., & Moore, B. S. (2022). Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria. Journal of the American Chemical Society, 144(21), 9372-9379. https://doi.org/10.1021/jacs.2c01424

Vancouver

Lima ST, Fallon TR, Cordoza JL, Chekan JR, Delbaje E, Hopiavuori AR et al. Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria. Journal of the American Chemical Society. 2022;144(21):9372-9379. https://doi.org/10.1021/jacs.2c01424

Author

Lima, Stella T. ; Fallon, Timothy R. ; Cordoza, Jennifer L. ; Chekan, Jonathan R. ; Delbaje, Endrews ; Hopiavuori, Austin R. ; Alvarenga, Danillo O. ; Wood, Steffaney M. ; Luhavaya, Hanna ; Baumgartner, Jackson T. ; Dörr, Felipe A. ; Etchegaray, Augusto ; Pinto, Ernani ; McKinnie, Shaun M. K. ; Fiore, Marli F. ; Moore, Bradley S. / Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria. In: Journal of the American Chemical Society. 2022 ; Vol. 144, No. 21. pp. 9372-9379.

Bibtex

@article{b24791cda8924f919a171ecc43485968,
title = "Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria",
abstract = "Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environmentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from l-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin ( gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate. ",
author = "Lima, {Stella T.} and Fallon, {Timothy R.} and Cordoza, {Jennifer L.} and Chekan, {Jonathan R.} and Endrews Delbaje and Hopiavuori, {Austin R.} and Alvarenga, {Danillo O.} and Wood, {Steffaney M.} and Hanna Luhavaya and Baumgartner, {Jackson T.} and D{\"o}rr, {Felipe A.} and Augusto Etchegaray and Ernani Pinto and McKinnie, {Shaun M. K.} and Fiore, {Marli F.} and Moore, {Bradley S.}",
year = "2022",
doi = "10.1021/jacs.2c01424",
language = "English",
volume = "144",
pages = "9372--9379",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "ACS Publications",
number = "21",

}

RIS

TY - JOUR

T1 - Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria

AU - Lima, Stella T.

AU - Fallon, Timothy R.

AU - Cordoza, Jennifer L.

AU - Chekan, Jonathan R.

AU - Delbaje, Endrews

AU - Hopiavuori, Austin R.

AU - Alvarenga, Danillo O.

AU - Wood, Steffaney M.

AU - Luhavaya, Hanna

AU - Baumgartner, Jackson T.

AU - Dörr, Felipe A.

AU - Etchegaray, Augusto

AU - Pinto, Ernani

AU - McKinnie, Shaun M. K.

AU - Fiore, Marli F.

AU - Moore, Bradley S.

PY - 2022

Y1 - 2022

N2 - Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environmentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from l-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin ( gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate.

AB - Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environmentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from l-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin ( gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate.

U2 - 10.1021/jacs.2c01424

DO - 10.1021/jacs.2c01424

M3 - Journal article

C2 - 35583956

VL - 144

SP - 9372

EP - 9379

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 21

ER -

ID: 308331737