Characterization and application of temperature micro-optodes for use in aquatic biology
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Characterization and application of temperature micro-optodes for use in aquatic biology. / Holst, Gerhard A.; Kuehl, Michael; Klimant, Ingo; Liebsch, Gregor; Kohls, Oliver.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2980, 1997, p. 164-170.Research output: Contribution to journal › Conference article › Research › peer-review
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TY - GEN
T1 - Characterization and application of temperature micro-optodes for use in aquatic biology
AU - Holst, Gerhard A.
AU - Kuehl, Michael
AU - Klimant, Ingo
AU - Liebsch, Gregor
AU - Kohls, Oliver
PY - 1997
Y1 - 1997
N2 - Benthic aquatic environments like biofilms or sediments are often investigation by measuring profiles of chemical or physical parameters at a high spatial resolution (< 50 micrometers ). This is necessary to understand e.g. transport processes and the biogeochemistry of the sediment water interface. A variety of electrochemical and optical microsensors has been developed and used for this purpose. In most of these applications the temperature of the investigated biofilms or sediments is assumed to be constant. However measurements with thermocouples of an appr. diameter of 300 micrometers have shown that this is not always the case for illuminated shallow water sediments and biofilms. We developed new microoptodes for measuring temperature distributions at a high spatial (< 50 micrometers ) and thermal (< 0.2 degree(s)C) resolution in aquatic systems. The new sensors are based on a fluorophore that is well known for its application in oxygen sensing-Ruthenium(II)- tris-1,10-phenantroline. Demas et al. (1992) discussed the possible use of highly luminescent transition metal complexes as temperature indicators. We have approached this idea from our experiences with ruthenium complexes as oxygen indicators. The first realized sensor consists of a closed microcapillary filled with an indicator solution and in inserted tapered optical fiber. The principle uses the temperature dependence of the fluorescence lifetime in the solution. To keep the solution oxygen free an oxygen scavenger is added to it. The change of the lifetime is detected by a special measuring device that uses a phase modulation technique.
AB - Benthic aquatic environments like biofilms or sediments are often investigation by measuring profiles of chemical or physical parameters at a high spatial resolution (< 50 micrometers ). This is necessary to understand e.g. transport processes and the biogeochemistry of the sediment water interface. A variety of electrochemical and optical microsensors has been developed and used for this purpose. In most of these applications the temperature of the investigated biofilms or sediments is assumed to be constant. However measurements with thermocouples of an appr. diameter of 300 micrometers have shown that this is not always the case for illuminated shallow water sediments and biofilms. We developed new microoptodes for measuring temperature distributions at a high spatial (< 50 micrometers ) and thermal (< 0.2 degree(s)C) resolution in aquatic systems. The new sensors are based on a fluorophore that is well known for its application in oxygen sensing-Ruthenium(II)- tris-1,10-phenantroline. Demas et al. (1992) discussed the possible use of highly luminescent transition metal complexes as temperature indicators. We have approached this idea from our experiences with ruthenium complexes as oxygen indicators. The first realized sensor consists of a closed microcapillary filled with an indicator solution and in inserted tapered optical fiber. The principle uses the temperature dependence of the fluorescence lifetime in the solution. To keep the solution oxygen free an oxygen scavenger is added to it. The change of the lifetime is detected by a special measuring device that uses a phase modulation technique.
KW - Fiber optic sensor
KW - Fibre optic sensor
KW - Luminescence lifetime
KW - Luminescence sensor
KW - Phase modulation
KW - Temperature microoptode
KW - Temperature microsensor
U2 - 10.1117/12.273525
DO - 10.1117/12.273525
M3 - Conference article
AN - SCOPUS:0001518331
VL - 2980
SP - 164
EP - 170
JO - Progress in Biomedical Optics and Imaging
JF - Progress in Biomedical Optics and Imaging
SN - 1605-7422
T2 - Advances in Fluorescence Sensing Technology III
Y2 - 9 February 1997 through 9 February 1997
ER -
ID: 201683205