Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Standard

Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains : Genomes in Flux. / Bahl, Martin Iain; Oregaard, Gunnar; Sørensen, Søren Johannes; Hansen, Lars H.

Horizontal Gene Transfer. ed. / Maria Boekels Gogarten; Johann Peter Gogarten; Lorraine C. Olendzenski. Vol. 532 Humana Press, 2009. p. 257-268 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Harvard

Bahl, MI, Oregaard, G, Sørensen, SJ & Hansen, LH 2009, Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux. in MB Gogarten, JP Gogarten & LC Olendzenski (eds), Horizontal Gene Transfer. vol. 532, Humana Press, Methods in Molecular Biology, pp. 257-268. https://doi.org/10.1007/978-1-60327-853-9_15

APA

Bahl, M. I., Oregaard, G., Sørensen, S. J., & Hansen, L. H. (2009). Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux. In M. B. Gogarten, J. P. Gogarten, & L. C. Olendzenski (Eds.), Horizontal Gene Transfer (Vol. 532, pp. 257-268). Humana Press. Methods in Molecular Biology https://doi.org/10.1007/978-1-60327-853-9_15

Vancouver

Bahl MI, Oregaard G, Sørensen SJ, Hansen LH. Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux. In Gogarten MB, Gogarten JP, Olendzenski LC, editors, Horizontal Gene Transfer. Vol. 532. Humana Press. 2009. p. 257-268. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-853-9_15

Author

Bahl, Martin Iain ; Oregaard, Gunnar ; Sørensen, Søren Johannes ; Hansen, Lars H. / Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains : Genomes in Flux. Horizontal Gene Transfer. editor / Maria Boekels Gogarten ; Johann Peter Gogarten ; Lorraine C. Olendzenski. Vol. 532 Humana Press, 2009. pp. 257-268 (Methods in Molecular Biology).

Bibtex

@inbook{3b2fdd501df111deb43e000ea68e967b,
title = "Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux",
abstract = "Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.",
author = "Bahl, {Martin Iain} and Gunnar Oregaard and S{\o}rensen, {S{\o}ren Johannes} and Hansen, {Lars H.}",
note = "Keywords Plasmid stability - green fluorescence protein - flow cytometry - plasmid-sensor strain - lac repressor",
year = "2009",
doi = "10.1007/978-1-60327-853-9_15",
language = "English",
isbn = "978-1-60327-852-2",
volume = "532",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "257--268",
editor = "Gogarten, {Maria Boekels} and Gogarten, {Johann Peter} and Olendzenski, {Lorraine C.}",
booktitle = "Horizontal Gene Transfer",
address = "United States",

}

RIS

TY - CHAP

T1 - Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains

T2 - Genomes in Flux

AU - Bahl, Martin Iain

AU - Oregaard, Gunnar

AU - Sørensen, Søren Johannes

AU - Hansen, Lars H.

N1 - Keywords Plasmid stability - green fluorescence protein - flow cytometry - plasmid-sensor strain - lac repressor

PY - 2009

Y1 - 2009

N2 - Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.

AB - Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.

U2 - 10.1007/978-1-60327-853-9_15

DO - 10.1007/978-1-60327-853-9_15

M3 - Book chapter

C2 - 19271190

SN - 978-1-60327-852-2

VL - 532

T3 - Methods in Molecular Biology

SP - 257

EP - 268

BT - Horizontal Gene Transfer

A2 - Gogarten, Maria Boekels

A2 - Gogarten, Johann Peter

A2 - Olendzenski, Lorraine C.

PB - Humana Press

ER -

ID: 11663620