Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene. / Bahl, Martin Iain; Hansen, Lars Hestbjerg; Sørensen, Søren Johannes.

In: FEMS Microbiology Letters, Vol. 253, No. 2, 2005, p. 201-205.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bahl, MI, Hansen, LH & Sørensen, SJ 2005, 'Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene', FEMS Microbiology Letters, vol. 253, no. 2, pp. 201-205. https://doi.org/10.1016/j.femsle.2005.09.034

APA

Bahl, M. I., Hansen, L. H., & Sørensen, S. J. (2005). Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene. FEMS Microbiology Letters, 253(2), 201-205. https://doi.org/10.1016/j.femsle.2005.09.034

Vancouver

Bahl MI, Hansen LH, Sørensen SJ. Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene. FEMS Microbiology Letters. 2005;253(2):201-205. https://doi.org/10.1016/j.femsle.2005.09.034

Author

Bahl, Martin Iain ; Hansen, Lars Hestbjerg ; Sørensen, Søren Johannes. / Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene. In: FEMS Microbiology Letters. 2005 ; Vol. 253, No. 2. pp. 201-205.

Bibtex

@article{29712fd074c311dbbee902004c4f4f50,
title = "Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene",
abstract = "An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.",
author = "Bahl, {Martin Iain} and Hansen, {Lars Hestbjerg} and S{\o}rensen, {S{\o}ren Johannes}",
note = "Keywords: Biosensor; Flow cytometry; Tetracycline",
year = "2005",
doi = "10.1016/j.femsle.2005.09.034",
language = "English",
volume = "253",
pages = "201--205",
journal = "F E M S Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene

AU - Bahl, Martin Iain

AU - Hansen, Lars Hestbjerg

AU - Sørensen, Søren Johannes

N1 - Keywords: Biosensor; Flow cytometry; Tetracycline

PY - 2005

Y1 - 2005

N2 - An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.

AB - An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.

U2 - 10.1016/j.femsle.2005.09.034

DO - 10.1016/j.femsle.2005.09.034

M3 - Journal article

C2 - 16239081

VL - 253

SP - 201

EP - 205

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 2

ER -

ID: 87541