Coping with copper: legacy effect of copper on potential activity of soil bacteria following a century of exposure
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Coping with copper : legacy effect of copper on potential activity of soil bacteria following a century of exposure. / Nunes, Ines Marques; Jacquiod, Samuel Jehan Auguste; Brejnrod, Asker Daniel; Holm, Peter Engelund; Johansen, Anders; Brandt, Kristian Koefoed; Priemé, Anders; Sørensen, Søren Johannes.
In: F E M S Microbiology Ecology, Vol. 92, No. 11, fiw175, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Coping with copper
T2 - legacy effect of copper on potential activity of soil bacteria following a century of exposure
AU - Nunes, Ines Marques
AU - Jacquiod, Samuel Jehan Auguste
AU - Brejnrod, Asker Daniel
AU - Holm, Peter Engelund
AU - Johansen, Anders
AU - Brandt, Kristian Koefoed
AU - Priemé, Anders
AU - Sørensen, Søren Johannes
N1 - © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2016
Y1 - 2016
N2 - Copper has been intensively used in industry and agriculture since mid-18(th) century and is currently accumulating in soils. We investigated the diversity of potential active bacteria by 16S rRNA gene transcript amplicon sequencing in a temperate grassland soil subjected to century-long exposure to normal (∼15 mg kg(-1)), high (∼450 mg kg(-1)) or extremely high (∼4500 mg kg(-1)) copper levels. Results showed that bioavailable copper had pronounced impacts on the structure of the transcriptionally active bacterial community, overruling other environmental factors (e.g. season and pH). As copper concentration increased, bacterial richness and evenness were negatively impacted, while distinct communities with an enhanced relative abundance of Nitrospira and Acidobacteria members and a lower representation of Verrucomicrobia, Proteobacteria and Actinobacteria were selected. Our analysis showed the presence of six functional response groups (FRGs), each consisting of bacterial taxa with similar tolerance response to copper. Furthermore, the use of FRGs revealed that specific taxa like the genus Nitrospira and several Acidobacteria groups could accurately predict the copper legacy burden in our system, suggesting a potential promising role as bioindicators of copper contamination in soils.
AB - Copper has been intensively used in industry and agriculture since mid-18(th) century and is currently accumulating in soils. We investigated the diversity of potential active bacteria by 16S rRNA gene transcript amplicon sequencing in a temperate grassland soil subjected to century-long exposure to normal (∼15 mg kg(-1)), high (∼450 mg kg(-1)) or extremely high (∼4500 mg kg(-1)) copper levels. Results showed that bioavailable copper had pronounced impacts on the structure of the transcriptionally active bacterial community, overruling other environmental factors (e.g. season and pH). As copper concentration increased, bacterial richness and evenness were negatively impacted, while distinct communities with an enhanced relative abundance of Nitrospira and Acidobacteria members and a lower representation of Verrucomicrobia, Proteobacteria and Actinobacteria were selected. Our analysis showed the presence of six functional response groups (FRGs), each consisting of bacterial taxa with similar tolerance response to copper. Furthermore, the use of FRGs revealed that specific taxa like the genus Nitrospira and several Acidobacteria groups could accurately predict the copper legacy burden in our system, suggesting a potential promising role as bioindicators of copper contamination in soils.
KW - Journal Article
U2 - 10.1093/femsec/fiw175
DO - 10.1093/femsec/fiw175
M3 - Journal article
C2 - 27543319
VL - 92
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 11
M1 - fiw175
ER -
ID: 165748635