Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere

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Standard

Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere. / Musovic, Sanin; Oregaard, Gunnar; Kroer, Niels; Sørensen, Søren J.

In: Applied and Environmental Microbiology, Vol. 72, No. 10, 2006, p. 6687-6692.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Musovic, S, Oregaard, G, Kroer, N & Sørensen, SJ 2006, 'Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere', Applied and Environmental Microbiology, vol. 72, no. 10, pp. 6687-6692. https://doi.org/10.1128/AEM.00013-06

APA

Musovic, S., Oregaard, G., Kroer, N., & Sørensen, S. J. (2006). Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere. Applied and Environmental Microbiology, 72(10), 6687-6692. https://doi.org/10.1128/AEM.00013-06

Vancouver

Musovic S, Oregaard G, Kroer N, Sørensen SJ. Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere. Applied and Environmental Microbiology. 2006;72(10):6687-6692. https://doi.org/10.1128/AEM.00013-06

Author

Musovic, Sanin ; Oregaard, Gunnar ; Kroer, Niels ; Sørensen, Søren J. / Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 10. pp. 6687-6692.

Bibtex

@article{eac3833e3bce4c298d99e1c929f9c9d8,
title = "Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere",
abstract = "The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 × 10 -2 ± 3.84 × 10-2. This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.",
author = "Sanin Musovic and Gunnar Oregaard and Niels Kroer and S{\o}rensen, {S{\o}ren J.}",
year = "2006",
doi = "10.1128/AEM.00013-06",
language = "English",
volume = "72",
pages = "6687--6692",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere

AU - Musovic, Sanin

AU - Oregaard, Gunnar

AU - Kroer, Niels

AU - Sørensen, Søren J.

PY - 2006

Y1 - 2006

N2 - The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 × 10 -2 ± 3.84 × 10-2. This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.

AB - The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 × 10 -2 ± 3.84 × 10-2. This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.

U2 - 10.1128/AEM.00013-06

DO - 10.1128/AEM.00013-06

M3 - Journal article

C2 - 17021220

AN - SCOPUS:33750051966

VL - 72

SP - 6687

EP - 6692

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -

ID: 214688006