Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements. / Pinilla-Redondo, Rafael; Shehreen, Saadlee; Marino, Nicole D.; Fagerlund, Robert D.; Brown, Chris M.; Sørensen, Søren J.; Fineran, Peter C.; Bondy-Denomy, Joseph.

In: Nature Communications, Vol. 11, 5652, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pinilla-Redondo, R, Shehreen, S, Marino, ND, Fagerlund, RD, Brown, CM, Sørensen, SJ, Fineran, PC & Bondy-Denomy, J 2020, 'Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements', Nature Communications, vol. 11, 5652. https://doi.org/10.1038/s41467-020-19415-3

APA

Pinilla-Redondo, R., Shehreen, S., Marino, N. D., Fagerlund, R. D., Brown, C. M., Sørensen, S. J., Fineran, P. C., & Bondy-Denomy, J. (2020). Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements. Nature Communications, 11, [5652]. https://doi.org/10.1038/s41467-020-19415-3

Vancouver

Pinilla-Redondo R, Shehreen S, Marino ND, Fagerlund RD, Brown CM, Sørensen SJ et al. Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements. Nature Communications. 2020;11. 5652. https://doi.org/10.1038/s41467-020-19415-3

Author

Pinilla-Redondo, Rafael ; Shehreen, Saadlee ; Marino, Nicole D. ; Fagerlund, Robert D. ; Brown, Chris M. ; Sørensen, Søren J. ; Fineran, Peter C. ; Bondy-Denomy, Joseph. / Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements. In: Nature Communications. 2020 ; Vol. 11.

Bibtex

@article{1f62c578659440fead2978c7353c1d74,
title = "Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements",
abstract = "Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems.",
author = "Rafael Pinilla-Redondo and Saadlee Shehreen and Marino, {Nicole D.} and Fagerlund, {Robert D.} and Brown, {Chris M.} and S{\o}rensen, {S{\o}ren J.} and Fineran, {Peter C.} and Joseph Bondy-Denomy",
year = "2020",
doi = "10.1038/s41467-020-19415-3",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

AU - Pinilla-Redondo, Rafael

AU - Shehreen, Saadlee

AU - Marino, Nicole D.

AU - Fagerlund, Robert D.

AU - Brown, Chris M.

AU - Sørensen, Søren J.

AU - Fineran, Peter C.

AU - Bondy-Denomy, Joseph

PY - 2020

Y1 - 2020

N2 - Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems.

AB - Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems.

U2 - 10.1038/s41467-020-19415-3

DO - 10.1038/s41467-020-19415-3

M3 - Journal article

C2 - 33159058

AN - SCOPUS:85095706172

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 5652

ER -

ID: 251737452