Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea

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Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea. / Holmfeldt, Karin; Dziallas, Claudia; Titelman, Josefin; Pohlmann, Kirsten; Grossart, Hans-Peter ; Riemann, Lasse.

In: Environmental Microbiology, Vol. 11, No. 8, 2009, p. 2042-2054.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Holmfeldt, K, Dziallas, C, Titelman, J, Pohlmann, K, Grossart, H-P & Riemann, L 2009, 'Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea', Environmental Microbiology, vol. 11, no. 8, pp. 2042-2054. https://doi.org/10.1111/j.1462-2920.2009.01925.x

APA

Holmfeldt, K., Dziallas, C., Titelman, J., Pohlmann, K., Grossart, H-P., & Riemann, L. (2009). Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea. Environmental Microbiology, 11(8), 2042-2054. https://doi.org/10.1111/j.1462-2920.2009.01925.x

Vancouver

Holmfeldt K, Dziallas C, Titelman J, Pohlmann K, Grossart H-P, Riemann L. Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea. Environmental Microbiology. 2009;11(8):2042-2054. https://doi.org/10.1111/j.1462-2920.2009.01925.x

Author

Holmfeldt, Karin ; Dziallas, Claudia ; Titelman, Josefin ; Pohlmann, Kirsten ; Grossart, Hans-Peter ; Riemann, Lasse. / Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea. In: Environmental Microbiology. 2009 ; Vol. 11, No. 8. pp. 2042-2054.

Bibtex

@article{77d9efb0c17511df825b000ea68e967b,
title = "Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea",
abstract = "Actinobacteria are highly abundant in pelagic freshwater habitats and also occur in estuarine environments such as the Baltic Sea. Because of gradients insalinity and other environmental variables estuaries offer natural systems for examining factors that determine Actinobacteria distribution. We studiedabundance and community structure of Bacteria and Actinobacteria along two transects in the northern Baltic Sea. Quantitative (CARD-FISH) and qualitative(DGGE and clone libraries) analyses of community composition were compared with environmental parameters. Actinobacteria accounted for 22–27% ofall bacteria and the abundance changed with temperature. Analysis of 549 actinobacterial 16S rRNA sequences from four clone libraries revealed a dominance of the freshwater clusters acI and acIV, and two new subclusters (acI-B scB-5 and acIV-E) were assigned. Whereas acI was present at all stations,occurrence of acII and acIV differed between stations and was related to dissolved organic carbon (DOC) and chlorophyll a (Chl a) respectively. The prevalence of the acI-A and acI-B subclusters changed in relation to total phosphorus (Tot-P) and Chl a respectively. Community structure of Bacteria and Actinobacteria differed between the river station and all other stations, responding to differences in DOC, Chl a and bacterial production. In contrast, the composition of active Actinobacteria (analysis based on reverselytranscribed RNA) changed in relation to salinity and Tot-P. Our study suggests an important ecological role of Actinobacteria in the brackish northernBaltic Sea. It highlights the need to address dynamics at the cluster or subcluster phylogenetic levels to gain insights into the factors regulating distribution and composition of Actinobacteria in aquatic environments.",
author = "Karin Holmfeldt and Claudia Dziallas and Josefin Titelman and Kirsten Pohlmann and Hans-Peter Grossart and Lasse Riemann",
year = "2009",
doi = "10.1111/j.1462-2920.2009.01925.x",
language = "English",
volume = "11",
pages = "2042--2054",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS

TY - JOUR

T1 - Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea

AU - Holmfeldt, Karin

AU - Dziallas, Claudia

AU - Titelman, Josefin

AU - Pohlmann, Kirsten

AU - Grossart, Hans-Peter

AU - Riemann, Lasse

PY - 2009

Y1 - 2009

N2 - Actinobacteria are highly abundant in pelagic freshwater habitats and also occur in estuarine environments such as the Baltic Sea. Because of gradients insalinity and other environmental variables estuaries offer natural systems for examining factors that determine Actinobacteria distribution. We studiedabundance and community structure of Bacteria and Actinobacteria along two transects in the northern Baltic Sea. Quantitative (CARD-FISH) and qualitative(DGGE and clone libraries) analyses of community composition were compared with environmental parameters. Actinobacteria accounted for 22–27% ofall bacteria and the abundance changed with temperature. Analysis of 549 actinobacterial 16S rRNA sequences from four clone libraries revealed a dominance of the freshwater clusters acI and acIV, and two new subclusters (acI-B scB-5 and acIV-E) were assigned. Whereas acI was present at all stations,occurrence of acII and acIV differed between stations and was related to dissolved organic carbon (DOC) and chlorophyll a (Chl a) respectively. The prevalence of the acI-A and acI-B subclusters changed in relation to total phosphorus (Tot-P) and Chl a respectively. Community structure of Bacteria and Actinobacteria differed between the river station and all other stations, responding to differences in DOC, Chl a and bacterial production. In contrast, the composition of active Actinobacteria (analysis based on reverselytranscribed RNA) changed in relation to salinity and Tot-P. Our study suggests an important ecological role of Actinobacteria in the brackish northernBaltic Sea. It highlights the need to address dynamics at the cluster or subcluster phylogenetic levels to gain insights into the factors regulating distribution and composition of Actinobacteria in aquatic environments.

AB - Actinobacteria are highly abundant in pelagic freshwater habitats and also occur in estuarine environments such as the Baltic Sea. Because of gradients insalinity and other environmental variables estuaries offer natural systems for examining factors that determine Actinobacteria distribution. We studiedabundance and community structure of Bacteria and Actinobacteria along two transects in the northern Baltic Sea. Quantitative (CARD-FISH) and qualitative(DGGE and clone libraries) analyses of community composition were compared with environmental parameters. Actinobacteria accounted for 22–27% ofall bacteria and the abundance changed with temperature. Analysis of 549 actinobacterial 16S rRNA sequences from four clone libraries revealed a dominance of the freshwater clusters acI and acIV, and two new subclusters (acI-B scB-5 and acIV-E) were assigned. Whereas acI was present at all stations,occurrence of acII and acIV differed between stations and was related to dissolved organic carbon (DOC) and chlorophyll a (Chl a) respectively. The prevalence of the acI-A and acI-B subclusters changed in relation to total phosphorus (Tot-P) and Chl a respectively. Community structure of Bacteria and Actinobacteria differed between the river station and all other stations, responding to differences in DOC, Chl a and bacterial production. In contrast, the composition of active Actinobacteria (analysis based on reverselytranscribed RNA) changed in relation to salinity and Tot-P. Our study suggests an important ecological role of Actinobacteria in the brackish northernBaltic Sea. It highlights the need to address dynamics at the cluster or subcluster phylogenetic levels to gain insights into the factors regulating distribution and composition of Actinobacteria in aquatic environments.

U2 - 10.1111/j.1462-2920.2009.01925.x

DO - 10.1111/j.1462-2920.2009.01925.x

M3 - Journal article

C2 - 19453610

VL - 11

SP - 2042

EP - 2054

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 8

ER -

ID: 22020434