Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia

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Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia. / Severin, Marc; Pedersen, Emma Lind; Borre, Magnus Thane; Axholm, Ida; Christiansen, Frederik Bendix; Ponniah, Muthulakshmi; Czaplinska, Dominika; Larsen, Tanja; Pardo, Luis Angel; Pedersen, Stine Falsig.

In: Journal of Cell Science, Vol. 136, No. 7, jcs260687, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Severin, M, Pedersen, EL, Borre, MT, Axholm, I, Christiansen, FB, Ponniah, M, Czaplinska, D, Larsen, T, Pardo, LA & Pedersen, SF 2023, 'Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia', Journal of Cell Science, vol. 136, no. 7, jcs260687. https://doi.org/10.1242/jcs.260687

APA

Severin, M., Pedersen, E. L., Borre, M. T., Axholm, I., Christiansen, F. B., Ponniah, M., Czaplinska, D., Larsen, T., Pardo, L. A., & Pedersen, S. F. (2023). Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia. Journal of Cell Science, 136(7), [jcs260687]. https://doi.org/10.1242/jcs.260687

Vancouver

Severin M, Pedersen EL, Borre MT, Axholm I, Christiansen FB, Ponniah M et al. Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia. Journal of Cell Science. 2023;136(7). jcs260687. https://doi.org/10.1242/jcs.260687

Author

Severin, Marc ; Pedersen, Emma Lind ; Borre, Magnus Thane ; Axholm, Ida ; Christiansen, Frederik Bendix ; Ponniah, Muthulakshmi ; Czaplinska, Dominika ; Larsen, Tanja ; Pardo, Luis Angel ; Pedersen, Stine Falsig. / Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia. In: Journal of Cell Science. 2023 ; Vol. 136, No. 7.

Bibtex

@article{5d306a5667b645c0beef38e3d8b0b62f,
title = "Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia",
abstract = "Finely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3- co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells. This localization was abolished by cell-cell contact disruption, and co-immunoprecipitation (co-IP) and proximity ligation (PLA) revealed NBCn1 interaction with E-cadherin and DLG1, linking it to adherens junctions and the Scribble complex. NBCn1 also interacted with RhoA and localized to lamellipodia and filopodia in migrating cells. Finally, analysis of native and GFP-tagged NBCn1 localization, subcellular fractionation, co-IP with Arl13B and CEP164, and PLA of NBCn1 and tubulin in mitotic spindles led to the surprising conclusion that NBCn1 additionally localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role of NBCn1 in cell cycle progression.",
keywords = "Acid-base transport, Cell polarity, Cell–cell adhesion, Mitosis, Primary cilium, Scribble complex",
author = "Marc Severin and Pedersen, {Emma Lind} and Borre, {Magnus Thane} and Ida Axholm and Christiansen, {Frederik Bendix} and Muthulakshmi Ponniah and Dominika Czaplinska and Tanja Larsen and Pardo, {Luis Angel} and Pedersen, {Stine Falsig}",
note = "Publisher Copyright: {\textcopyright} 2023. Published by The Company of Biologists Ltd.",
year = "2023",
doi = "10.1242/jcs.260687",
language = "English",
volume = "136",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",
number = "7",

}

RIS

TY - JOUR

T1 - Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia

AU - Severin, Marc

AU - Pedersen, Emma Lind

AU - Borre, Magnus Thane

AU - Axholm, Ida

AU - Christiansen, Frederik Bendix

AU - Ponniah, Muthulakshmi

AU - Czaplinska, Dominika

AU - Larsen, Tanja

AU - Pardo, Luis Angel

AU - Pedersen, Stine Falsig

N1 - Publisher Copyright: © 2023. Published by The Company of Biologists Ltd.

PY - 2023

Y1 - 2023

N2 - Finely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3- co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells. This localization was abolished by cell-cell contact disruption, and co-immunoprecipitation (co-IP) and proximity ligation (PLA) revealed NBCn1 interaction with E-cadherin and DLG1, linking it to adherens junctions and the Scribble complex. NBCn1 also interacted with RhoA and localized to lamellipodia and filopodia in migrating cells. Finally, analysis of native and GFP-tagged NBCn1 localization, subcellular fractionation, co-IP with Arl13B and CEP164, and PLA of NBCn1 and tubulin in mitotic spindles led to the surprising conclusion that NBCn1 additionally localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role of NBCn1 in cell cycle progression.

AB - Finely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3- co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells. This localization was abolished by cell-cell contact disruption, and co-immunoprecipitation (co-IP) and proximity ligation (PLA) revealed NBCn1 interaction with E-cadherin and DLG1, linking it to adherens junctions and the Scribble complex. NBCn1 also interacted with RhoA and localized to lamellipodia and filopodia in migrating cells. Finally, analysis of native and GFP-tagged NBCn1 localization, subcellular fractionation, co-IP with Arl13B and CEP164, and PLA of NBCn1 and tubulin in mitotic spindles led to the surprising conclusion that NBCn1 additionally localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role of NBCn1 in cell cycle progression.

KW - Acid-base transport

KW - Cell polarity

KW - Cell–cell adhesion

KW - Mitosis

KW - Primary cilium

KW - Scribble complex

U2 - 10.1242/jcs.260687

DO - 10.1242/jcs.260687

M3 - Journal article

C2 - 37039101

AN - SCOPUS:85152111149

VL - 136

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 7

M1 - jcs260687

ER -

ID: 344810685