Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. / Van Walree, Cornelis A.; Sakuragi, Yumiko; Steensgaard, Dorte B.; Bösinger, Carola S.; Frigaard, Niels-Ulrik; Cox, Raymond P.; Holzwart, Alfred R.; Miller, Mette.

In: Photochemistry and Photobiology, Vol. 69, No. 3, 01.01.1999, p. 322-328.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Van Walree, CA, Sakuragi, Y, Steensgaard, DB, Bösinger, CS, Frigaard, N-U, Cox, RP, Holzwart, AR & Miller, M 1999, 'Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides', Photochemistry and Photobiology, vol. 69, no. 3, pp. 322-328. https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

APA

Van Walree, C. A., Sakuragi, Y., Steensgaard, D. B., Bösinger, C. S., Frigaard, N-U., Cox, R. P., Holzwart, A. R., & Miller, M. (1999). Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. Photochemistry and Photobiology, 69(3), 322-328. https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

Vancouver

Van Walree CA, Sakuragi Y, Steensgaard DB, Bösinger CS, Frigaard N-U, Cox RP et al. Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. Photochemistry and Photobiology. 1999 Jan 1;69(3):322-328. https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

Author

Van Walree, Cornelis A. ; Sakuragi, Yumiko ; Steensgaard, Dorte B. ; Bösinger, Carola S. ; Frigaard, Niels-Ulrik ; Cox, Raymond P. ; Holzwart, Alfred R. ; Miller, Mette. / Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. In: Photochemistry and Photobiology. 1999 ; Vol. 69, No. 3. pp. 322-328.

Bibtex

@article{1cfd4c7037a94e1d8c43c1720a88ef89,
title = "Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides",
abstract = "Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.",
author = "{Van Walree}, {Cornelis A.} and Yumiko Sakuragi and Steensgaard, {Dorte B.} and B{\"o}singer, {Carola S.} and Niels-Ulrik Frigaard and Cox, {Raymond P.} and Holzwart, {Alfred R.} and Mette Miller",
year = "1999",
month = jan,
day = "1",
doi = "10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2",
language = "English",
volume = "69",
pages = "322--328",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides

AU - Van Walree, Cornelis A.

AU - Sakuragi, Yumiko

AU - Steensgaard, Dorte B.

AU - Bösinger, Carola S.

AU - Frigaard, Niels-Ulrik

AU - Cox, Raymond P.

AU - Holzwart, Alfred R.

AU - Miller, Mette

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.

AB - Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.

UR - http://www.scopus.com/inward/record.url?scp=0001451488&partnerID=8YFLogxK

U2 - 10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

DO - 10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

M3 - Journal article

AN - SCOPUS:0001451488

VL - 69

SP - 322

EP - 328

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 3

ER -

ID: 198772239