Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata. / Snyder, S.; Nadler, L.E.; Bayley, J.S.; Svendsen, Morten Bo Søndergaard; Johansen, J.L.; Domenici, P.; Steffensen, John Fleng.
In: Journal of Fish Biology, Vol. 88, No. 1, 2016, p. 252-264.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata
AU - Snyder, S.
AU - Nadler, L.E.
AU - Bayley, J.S.
AU - Svendsen, Morten Bo Søndergaard
AU - Johansen, J.L.
AU - Domenici, P.
AU - Steffensen, John Fleng
N1 - © 2016 The Fisheries Society of the British Isles.
PY - 2016
Y1 - 2016
N2 - This study compares the critical oxygen saturation (O2 crit ) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.
AB - This study compares the critical oxygen saturation (O2 crit ) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.
U2 - 10.1111/jfb.12837
DO - 10.1111/jfb.12837
M3 - Journal article
C2 - 26768977
VL - 88
SP - 252
EP - 264
JO - Journal of Fish Biology
JF - Journal of Fish Biology
SN - 0022-1112
IS - 1
ER -
ID: 153592435