Expression of calmodulin and calmodulin binding proteins in rat fibroblasts stably transfected with protein kinase C and oncogenes
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Expression of calmodulin and calmodulin binding proteins in rat fibroblasts stably transfected with protein kinase C and oncogenes. / Ye, Q; Wei, Y; Fischer, R; Borner, C; Berchtold, M W.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1359, No. 1, 1997, p. 89-96.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Expression of calmodulin and calmodulin binding proteins in rat fibroblasts stably transfected with protein kinase C and oncogenes
AU - Ye, Q
AU - Wei, Y
AU - Fischer, R
AU - Borner, C
AU - Berchtold, M W
N1 - Keywords: Animals; Blotting, Northern; Calcium; Calmodulin; Calmodulin-Binding Proteins; Cell Line; Cell Line, Transformed; Cell Transformation, Neoplastic; DNA Probes; Egtazic Acid; Fibroblasts; Gene Expression Regulation; Isoenzymes; Oncogenes; Protein Kinase C; RNA, Messenger; Rats; Transcription, Genetic; Transfection
PY - 1997
Y1 - 1997
N2 - Molecular mechanisms leading to elevated calmodulin (CaM) expression in cancer have not yet been discovered. We have quantitated the levels of transcripts derived from all three CaM genes in a variety of the same origin rat fibroblasts transformed with oncogenes in combination with gene for protein kinase C using Northern blot analysis with three CaM gene specific cDNA probes. Five species of CaM mRNA were detected in all these cells. Surprisingly many of the investigated cell lines exhibited a decreased content of all CaM mRNAs as compared to control cells with CaMI and CaMII transcripts showing the most pronounced alterations. In contrast, CaM protein levels were increased in all these cell lines as determined by a radioimmunoassay. These results suggest that oncogenic up-regulation of CaM synthesis takes place posttranscriptionally. Several CaM binding proteins were found at different concentrations in the studied cell lines depending on the oncogenes used for transformation. However, CaM overexpression does not seem to affect the overall levels of CaM binding proteins.
AB - Molecular mechanisms leading to elevated calmodulin (CaM) expression in cancer have not yet been discovered. We have quantitated the levels of transcripts derived from all three CaM genes in a variety of the same origin rat fibroblasts transformed with oncogenes in combination with gene for protein kinase C using Northern blot analysis with three CaM gene specific cDNA probes. Five species of CaM mRNA were detected in all these cells. Surprisingly many of the investigated cell lines exhibited a decreased content of all CaM mRNAs as compared to control cells with CaMI and CaMII transcripts showing the most pronounced alterations. In contrast, CaM protein levels were increased in all these cell lines as determined by a radioimmunoassay. These results suggest that oncogenic up-regulation of CaM synthesis takes place posttranscriptionally. Several CaM binding proteins were found at different concentrations in the studied cell lines depending on the oncogenes used for transformation. However, CaM overexpression does not seem to affect the overall levels of CaM binding proteins.
U2 - 10.1016/S0167-4889(97)00086-4
DO - 10.1016/S0167-4889(97)00086-4
M3 - Journal article
C2 - 9398089
VL - 1359
SP - 89
EP - 96
JO - B B A - Molecular Cell Research
JF - B B A - Molecular Cell Research
SN - 0167-4889
IS - 1
ER -
ID: 11175089