Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

Research output: Contribution to journal › Journal article › Research › peer-review

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Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation. / Frigaard, Niels-Ulrik; Sakuragi, Yumiko; Bryant, Donald A.

In: Methods in Molecular Biology, Vol. 274, 2004, p. 325-340.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Frigaard, N-U, Sakuragi, Y & Bryant, DA 2004, 'Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation', Methods in Molecular Biology, vol. 274, pp. 325-340. https://doi.org/10.1385/1-59259-799-8:325

APA

Frigaard, N-U., Sakuragi, Y., & Bryant, D. A. (2004). Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation. Methods in Molecular Biology, 274, 325-340. https://doi.org/10.1385/1-59259-799-8:325

Vancouver

Frigaard N-U, Sakuragi Y, Bryant DA. Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation. Methods in Molecular Biology. 2004;274:325-340. https://doi.org/10.1385/1-59259-799-8:325

Author

Frigaard, Niels-Ulrik ; Sakuragi, Yumiko ; Bryant, Donald A. / Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation. In: Methods in Molecular Biology. 2004 ; Vol. 274. pp. 325-340.

Bibtex

@article{d2725c40962511de8bc9000ea68e967b,
title = "Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation",
abstract = "Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.",
author = "Niels-Ulrik Frigaard and Yumiko Sakuragi and Bryant, {Donald A}",
note = "Keywords: Chlorobium; Cyanobacteria; DNA, Bacterial; Gene Silencing; Mutagenesis; Transformation, Bacterial",
year = "2004",
doi = "10.1385/1-59259-799-8:325",
language = "English",
volume = "274",
pages = "325--340",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

AU - Frigaard, Niels-Ulrik

AU - Sakuragi, Yumiko

AU - Bryant, Donald A

N1 - Keywords: Chlorobium; Cyanobacteria; DNA, Bacterial; Gene Silencing; Mutagenesis; Transformation, Bacterial

PY - 2004

Y1 - 2004

N2 - Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.

AB - Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.

U2 - 10.1385/1-59259-799-8:325

DO - 10.1385/1-59259-799-8:325

M3 - Journal article

C2 - 15187290

VL - 274

SP - 325

EP - 340

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 14095443