Genetic analysis of Schizosaccharomyces pombe

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Genetic analysis of Schizosaccharomyces pombe. / Ekwall, Karl; Thon, Genevieve.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 8, 08.2017, p. 593-605.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ekwall, K & Thon, G 2017, 'Genetic analysis of Schizosaccharomyces pombe', Cold Spring Harbor Protocols, vol. 2017, no. 8, pp. 593-605. https://doi.org/10.1101/pdb.top079772

APA

Ekwall, K., & Thon, G. (2017). Genetic analysis of Schizosaccharomyces pombe. Cold Spring Harbor Protocols, 2017(8), 593-605. https://doi.org/10.1101/pdb.top079772

Vancouver

Ekwall K, Thon G. Genetic analysis of Schizosaccharomyces pombe. Cold Spring Harbor Protocols. 2017 Aug;2017(8):593-605. https://doi.org/10.1101/pdb.top079772

Author

Ekwall, Karl ; Thon, Genevieve. / Genetic analysis of Schizosaccharomyces pombe. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 8. pp. 593-605.

Bibtex

@article{0501bfb7614a4d188cd9469650c0d02c,
title = "Genetic analysis of Schizosaccharomyces pombe",
abstract = "In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism. We describe its mating-type system, how to perform genetic crosses and methods for selecting and propagating diploids. We discuss the relative merits of tetrad dissection and random spore preparation in strain construction and genetic analyses. Finally, we present several types of mutant screens, with an evaluation of their respective strengths and limitations in the light of emerging technologies such as next-generation sequencing.",
author = "Karl Ekwall and Genevieve Thon",
year = "2017",
month = aug,
doi = "10.1101/pdb.top079772",
language = "English",
volume = "2017",
pages = "593--605",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "8",

}

RIS

TY - JOUR

T1 - Genetic analysis of Schizosaccharomyces pombe

AU - Ekwall, Karl

AU - Thon, Genevieve

PY - 2017/8

Y1 - 2017/8

N2 - In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism. We describe its mating-type system, how to perform genetic crosses and methods for selecting and propagating diploids. We discuss the relative merits of tetrad dissection and random spore preparation in strain construction and genetic analyses. Finally, we present several types of mutant screens, with an evaluation of their respective strengths and limitations in the light of emerging technologies such as next-generation sequencing.

AB - In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism. We describe its mating-type system, how to perform genetic crosses and methods for selecting and propagating diploids. We discuss the relative merits of tetrad dissection and random spore preparation in strain construction and genetic analyses. Finally, we present several types of mutant screens, with an evaluation of their respective strengths and limitations in the light of emerging technologies such as next-generation sequencing.

UR - http://www.scopus.com/inward/record.url?scp=85026808638&partnerID=8YFLogxK

U2 - 10.1101/pdb.top079772

DO - 10.1101/pdb.top079772

M3 - Journal article

C2 - 28765303

AN - SCOPUS:85026808638

VL - 2017

SP - 593

EP - 605

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 8

ER -

ID: 184072086