Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms. / Macedo, Goncalo; Olesen, Asmus K.; Maccario, Lorrie; Leal, Lucia Hernandez; v. d. Maas, Peter; Heederik, Dick; Mevius, Dik; Sørensen, Søren J.; Schmitt, Heike.

In: Environmental Science & Technology, Vol. 56, No. 16, 2022, p. 11398-11408.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Macedo, G, Olesen, AK, Maccario, L, Leal, LH, v. d. Maas, P, Heederik, D, Mevius, D, Sørensen, SJ & Schmitt, H 2022, 'Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms', Environmental Science & Technology, vol. 56, no. 16, pp. 11398-11408. https://doi.org/10.1021/acs.est.2c02686

APA

Macedo, G., Olesen, A. K., Maccario, L., Leal, L. H., v. d. Maas, P., Heederik, D., Mevius, D., Sørensen, S. J., & Schmitt, H. (2022). Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms. Environmental Science & Technology, 56(16), 11398-11408. https://doi.org/10.1021/acs.est.2c02686

Vancouver

Macedo G, Olesen AK, Maccario L, Leal LH, v. d. Maas P, Heederik D et al. Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms. Environmental Science & Technology. 2022;56(16):11398-11408. https://doi.org/10.1021/acs.est.2c02686

Author

Macedo, Goncalo ; Olesen, Asmus K. ; Maccario, Lorrie ; Leal, Lucia Hernandez ; v. d. Maas, Peter ; Heederik, Dick ; Mevius, Dik ; Sørensen, Søren J. ; Schmitt, Heike. / Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms. In: Environmental Science & Technology. 2022 ; Vol. 56, No. 16. pp. 11398-11408.

Bibtex

@article{a8b423bfe61f44848a56c7d0bb1c4a8e,
title = "Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms",
abstract = "The quantification and identification of new plasmid-acquiring bacteria in representative mating conditions is critical to characterize the risk of horizontal gene transfer in the environment. This study aimed to quantify conjugation events resulting from manure application to soils and identify the transconjugants resulting from these events. Conjugation was quantified at multiple time points by plating and flow cytometry, and the transconjugants were recovered by fluorescence-activated cell sorting and identified by 16S rRNA sequencing. Overall, transconjugants were only observed within the first 4 days after manure application and at values close to the detection limits of this experimental system (1.00-2.49 log CFU/g of manured soil, ranging between 10(-5) and 10(-4) transconjugants-to-donor ratios). In the pool of recovered transconjugants, we found amplicon sequence variants (ASVs) of genera whose origin was traced to soils (Bacillus and Nocardioides) and manure (Comamonas and Rahnella). This work showed that gene transfer from fecal to soil bacteria occurred despite the less-than-optimal conditions faced by manure bacteria when transferred to soils, but these events were rare, mainly happened shortly after manure application, and the plasmid did not colonize the soil community. This study provides important information to determine the risks of AMR spread via manure application.",
keywords = "lateral gene transfer, antibiotic resistance gene, soil microbiome, mating, cattle manure, ANTIBIOTIC-RESISTANCE GENES, ACINETOBACTER-CALCOACETICUS, NATURAL TRANSFORMATION, HOST-RANGE, IN-SITU, WASTE, DNA, BACTERIOPHAGES, PERMISSIVENESS, FERTILIZATION",
author = "Goncalo Macedo and Olesen, {Asmus K.} and Lorrie Maccario and Leal, {Lucia Hernandez} and {v. d. Maas}, Peter and Dick Heederik and Dik Mevius and S{\o}rensen, {S{\o}ren J.} and Heike Schmitt",
year = "2022",
doi = "10.1021/acs.est.2c02686",
language = "English",
volume = "56",
pages = "11398--11408",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "16",

}

RIS

TY - JOUR

T1 - Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by Escherichia coli through Manure Amendment in Soil Microcosms

AU - Macedo, Goncalo

AU - Olesen, Asmus K.

AU - Maccario, Lorrie

AU - Leal, Lucia Hernandez

AU - v. d. Maas, Peter

AU - Heederik, Dick

AU - Mevius, Dik

AU - Sørensen, Søren J.

AU - Schmitt, Heike

PY - 2022

Y1 - 2022

N2 - The quantification and identification of new plasmid-acquiring bacteria in representative mating conditions is critical to characterize the risk of horizontal gene transfer in the environment. This study aimed to quantify conjugation events resulting from manure application to soils and identify the transconjugants resulting from these events. Conjugation was quantified at multiple time points by plating and flow cytometry, and the transconjugants were recovered by fluorescence-activated cell sorting and identified by 16S rRNA sequencing. Overall, transconjugants were only observed within the first 4 days after manure application and at values close to the detection limits of this experimental system (1.00-2.49 log CFU/g of manured soil, ranging between 10(-5) and 10(-4) transconjugants-to-donor ratios). In the pool of recovered transconjugants, we found amplicon sequence variants (ASVs) of genera whose origin was traced to soils (Bacillus and Nocardioides) and manure (Comamonas and Rahnella). This work showed that gene transfer from fecal to soil bacteria occurred despite the less-than-optimal conditions faced by manure bacteria when transferred to soils, but these events were rare, mainly happened shortly after manure application, and the plasmid did not colonize the soil community. This study provides important information to determine the risks of AMR spread via manure application.

AB - The quantification and identification of new plasmid-acquiring bacteria in representative mating conditions is critical to characterize the risk of horizontal gene transfer in the environment. This study aimed to quantify conjugation events resulting from manure application to soils and identify the transconjugants resulting from these events. Conjugation was quantified at multiple time points by plating and flow cytometry, and the transconjugants were recovered by fluorescence-activated cell sorting and identified by 16S rRNA sequencing. Overall, transconjugants were only observed within the first 4 days after manure application and at values close to the detection limits of this experimental system (1.00-2.49 log CFU/g of manured soil, ranging between 10(-5) and 10(-4) transconjugants-to-donor ratios). In the pool of recovered transconjugants, we found amplicon sequence variants (ASVs) of genera whose origin was traced to soils (Bacillus and Nocardioides) and manure (Comamonas and Rahnella). This work showed that gene transfer from fecal to soil bacteria occurred despite the less-than-optimal conditions faced by manure bacteria when transferred to soils, but these events were rare, mainly happened shortly after manure application, and the plasmid did not colonize the soil community. This study provides important information to determine the risks of AMR spread via manure application.

KW - lateral gene transfer

KW - antibiotic resistance gene

KW - soil microbiome

KW - mating

KW - cattle manure

KW - ANTIBIOTIC-RESISTANCE GENES

KW - ACINETOBACTER-CALCOACETICUS

KW - NATURAL TRANSFORMATION

KW - HOST-RANGE

KW - IN-SITU

KW - WASTE

KW - DNA

KW - BACTERIOPHAGES

KW - PERMISSIVENESS

KW - FERTILIZATION

U2 - 10.1021/acs.est.2c02686

DO - 10.1021/acs.est.2c02686

M3 - Journal article

C2 - 35896060

VL - 56

SP - 11398

EP - 11408

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 16

ER -

ID: 316117001