Hypoxia as a physiological cue and pathological stress for coral larvae
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Hypoxia as a physiological cue and pathological stress for coral larvae. / Alderdice, Rachel; Pernice, Mathieu; Cárdenas, Anny; Hughes, David J.; Harrison, Peter L.; Boulotte, Nadine; Chartrand, Katie; Kühl, Michael; Suggett, David J.; Voolstra, Christian R.
In: Molecular Ecology, Vol. 31, No. 2, 2022, p. 571-587.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Hypoxia as a physiological cue and pathological stress for coral larvae
AU - Alderdice, Rachel
AU - Pernice, Mathieu
AU - Cárdenas, Anny
AU - Hughes, David J.
AU - Harrison, Peter L.
AU - Boulotte, Nadine
AU - Chartrand, Katie
AU - Kühl, Michael
AU - Suggett, David J.
AU - Voolstra, Christian R.
N1 - Publisher Copyright: © 2021 The Authors. Molecular Ecology published by John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - Ocean deoxygenation events are intensifying worldwide and can rapidly drive adult corals into a state of metabolic crisis and bleaching-induced mortality, but whether coral larvae are subject to similar stress remains untested. We experimentally exposed apo-symbiotic coral larvae of Acropora selago to deoxygenation stress with subsequent reoxygenation aligned to their night-day light cycle, and followed their gene expression using RNA-Seq. After 12 h of deoxygenation stress (~2 mg O2/L), coral planulae demonstrated a low expression of HIF-targeted hypoxia response genes concomitant with a significantly high expression of PHD2 (a promoter of HIFα proteasomal degradation), similar to corresponding adult corals. Despite exhibiting a consistent swimming phenotype compared to control samples, the differential gene expression observed in planulae exposed to deoxygenation-reoxygenation suggests a disruption of pathways involved in developmental regulation, mitochondrial activity, lipid metabolism, and O2-sensitive epigenetic regulators. Importantly, we found that treated larvae exhibited a disruption in the expression of conserved HIF-targeted developmental regulators, for example, Homeobox (HOX) genes, corroborating how changes in external oxygen levels can affect animal development. We discuss how the observed deoxygenation responses may be indicative of a possible acclimation response or alternatively may imply negative latent impacts for coral larval fitness.
AB - Ocean deoxygenation events are intensifying worldwide and can rapidly drive adult corals into a state of metabolic crisis and bleaching-induced mortality, but whether coral larvae are subject to similar stress remains untested. We experimentally exposed apo-symbiotic coral larvae of Acropora selago to deoxygenation stress with subsequent reoxygenation aligned to their night-day light cycle, and followed their gene expression using RNA-Seq. After 12 h of deoxygenation stress (~2 mg O2/L), coral planulae demonstrated a low expression of HIF-targeted hypoxia response genes concomitant with a significantly high expression of PHD2 (a promoter of HIFα proteasomal degradation), similar to corresponding adult corals. Despite exhibiting a consistent swimming phenotype compared to control samples, the differential gene expression observed in planulae exposed to deoxygenation-reoxygenation suggests a disruption of pathways involved in developmental regulation, mitochondrial activity, lipid metabolism, and O2-sensitive epigenetic regulators. Importantly, we found that treated larvae exhibited a disruption in the expression of conserved HIF-targeted developmental regulators, for example, Homeobox (HOX) genes, corroborating how changes in external oxygen levels can affect animal development. We discuss how the observed deoxygenation responses may be indicative of a possible acclimation response or alternatively may imply negative latent impacts for coral larval fitness.
KW - coral
KW - development
KW - gene expression profiling
KW - hypoxia
KW - RNA-Seq
U2 - 10.1111/mec.16259
DO - 10.1111/mec.16259
M3 - Journal article
C2 - 34716959
AN - SCOPUS:85119261632
VL - 31
SP - 571
EP - 587
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 2
ER -
ID: 286498648