Identification of miRNA targets with stable isotope labeling by amino acids in cell culture.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Identification of miRNA targets with stable isotope labeling by amino acids in cell culture. / Vinther, Jeppe; Hedegaard, Mads Marquardt; Gardner, Paul Phillip; Andersen, Jens S.; Arctander, Peter.

In: Nucleic Acids Research, Vol. 34, No. 16, 2006, p. e107.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vinther, J, Hedegaard, MM, Gardner, PP, Andersen, JS & Arctander, P 2006, 'Identification of miRNA targets with stable isotope labeling by amino acids in cell culture.', Nucleic Acids Research, vol. 34, no. 16, pp. e107. https://doi.org/10.1093/nar/gkl590

APA

Vinther, J., Hedegaard, M. M., Gardner, P. P., Andersen, J. S., & Arctander, P. (2006). Identification of miRNA targets with stable isotope labeling by amino acids in cell culture. Nucleic Acids Research, 34(16), e107. https://doi.org/10.1093/nar/gkl590

Vancouver

Vinther J, Hedegaard MM, Gardner PP, Andersen JS, Arctander P. Identification of miRNA targets with stable isotope labeling by amino acids in cell culture. Nucleic Acids Research. 2006;34(16):e107. https://doi.org/10.1093/nar/gkl590

Author

Vinther, Jeppe ; Hedegaard, Mads Marquardt ; Gardner, Paul Phillip ; Andersen, Jens S. ; Arctander, Peter. / Identification of miRNA targets with stable isotope labeling by amino acids in cell culture. In: Nucleic Acids Research. 2006 ; Vol. 34, No. 16. pp. e107.

Bibtex

@article{e4588e30d94211dcbee902004c4f4f50,
title = "Identification of miRNA targets with stable isotope labeling by amino acids in cell culture.",
abstract = "miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3'-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins. ",
author = "Jeppe Vinther and Hedegaard, {Mads Marquardt} and Gardner, {Paul Phillip} and Andersen, {Jens S.} and Peter Arctander",
year = "2006",
doi = "10.1093/nar/gkl590",
language = "English",
volume = "34",
pages = "e107",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "16",

}

RIS

TY - JOUR

T1 - Identification of miRNA targets with stable isotope labeling by amino acids in cell culture.

AU - Vinther, Jeppe

AU - Hedegaard, Mads Marquardt

AU - Gardner, Paul Phillip

AU - Andersen, Jens S.

AU - Arctander, Peter

PY - 2006

Y1 - 2006

N2 - miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3'-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins.

AB - miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3'-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins.

U2 - 10.1093/nar/gkl590

DO - 10.1093/nar/gkl590

M3 - Journal article

VL - 34

SP - e107

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 16

ER -

ID: 2704142