In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS
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In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS. / Jensen, Sheila I; Steunou, Anne-Soisig; Bhaya, Devaki; Kühl, Michael; Grossman, Arthur R.
In: I S M E Journal, Vol. 5, No. 2, 2011, p. 317-28.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS
AU - Jensen, Sheila I
AU - Steunou, Anne-Soisig
AU - Bhaya, Devaki
AU - Kühl, Michael
AU - Grossman, Arthur R
PY - 2011
Y1 - 2011
N2 - The relative abundance of transcripts encoding proteins involved in inorganic carbon concentrating mechanisms (CCM), detoxification of reactive oxygen species (ROS) and photosynthesis in the thermophilic cyanobacterium Synechococcus OS-B' was measured in hot spring microbial mats over two diel cycles, and was coupled with in situ determinations of incoming irradiance and microenvironmental dynamics of O(2) and pH. Fluctuations in pH and O(2) in the mats were largely driven by the diel cycle of solar irradiance, with a pH variation from ~7.0 to ~9.5, and O(2) levels ranging from anoxia to supersaturation during night and day, respectively. Levels of various transcripts from mat cyanobacteria revealed several patterns that correlated with incident irradiance, O(2) and pH within the mat matrix. Transcript abundances for most genes increased during the morning dark-light transition. Some transcripts remained at a near constant level throughout the light period, whereas others showed an additional increase in abundance as the mat underwent transition from low-to-high light (potentially reflecting changes in O(2) concentration and pH), followed by either a decreased abundance in the early afternoon, or a gradual decline during the early afternoon and into the evening. One specific transcipt, psbA1, was the lowest during mid-day under high irradiance and increased when the light levels declined. We discuss these complex in situ transcriptional patterns with respect to environmental and endogenous cues that might impact and regulate transcription over the diel cycle.
AB - The relative abundance of transcripts encoding proteins involved in inorganic carbon concentrating mechanisms (CCM), detoxification of reactive oxygen species (ROS) and photosynthesis in the thermophilic cyanobacterium Synechococcus OS-B' was measured in hot spring microbial mats over two diel cycles, and was coupled with in situ determinations of incoming irradiance and microenvironmental dynamics of O(2) and pH. Fluctuations in pH and O(2) in the mats were largely driven by the diel cycle of solar irradiance, with a pH variation from ~7.0 to ~9.5, and O(2) levels ranging from anoxia to supersaturation during night and day, respectively. Levels of various transcripts from mat cyanobacteria revealed several patterns that correlated with incident irradiance, O(2) and pH within the mat matrix. Transcript abundances for most genes increased during the morning dark-light transition. Some transcripts remained at a near constant level throughout the light period, whereas others showed an additional increase in abundance as the mat underwent transition from low-to-high light (potentially reflecting changes in O(2) concentration and pH), followed by either a decreased abundance in the early afternoon, or a gradual decline during the early afternoon and into the evening. One specific transcipt, psbA1, was the lowest during mid-day under high irradiance and increased when the light levels declined. We discuss these complex in situ transcriptional patterns with respect to environmental and endogenous cues that might impact and regulate transcription over the diel cycle.
KW - Carbon
KW - Carbon Dioxide
KW - Cyanobacteria
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Bacterial
KW - Hot Springs
KW - Hydrogen-Ion Concentration
KW - Oxygen
KW - Photosynthesis
KW - Reactive Oxygen Species
KW - Solar Energy
KW - Synechococcus
KW - Water Microbiology
U2 - 10.1038/ismej.2010.131
DO - 10.1038/ismej.2010.131
M3 - Journal article
C2 - 20740024
VL - 5
SP - 317
EP - 328
JO - I S M E Journal
JF - I S M E Journal
SN - 1751-7362
IS - 2
ER -
ID: 33224836