In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. / Nielsen, Jakob Toudahl; Kulminskaya, Natalia V.; Bjerring, Morten; Linnanto, Juha M; Rätsep, Margus; Pedersen, Marie Østergaard; Lambrev, Petar H.; Dorogi, Márta; Garab, Győző; Thomsen, Karen; Jegerschöld, Caroline; Frigaard, Niels-Ulrik; Lindahl, Martin; Nielsen, Niels Chr.

In: Nature Communications, Vol. 7, 12454, 2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, JT, Kulminskaya, NV, Bjerring, M, Linnanto, JM, Rätsep, M, Pedersen, MØ, Lambrev, PH, Dorogi, M, Garab, G, Thomsen, K, Jegerschöld, C, Frigaard, N-U, Lindahl, M & Nielsen, NC 2016, 'In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum', Nature Communications, vol. 7, 12454. https://doi.org/10.1038/ncomms12454

APA

Nielsen, J. T., Kulminskaya, N. V., Bjerring, M., Linnanto, J. M., Rätsep, M., Pedersen, M. Ø., Lambrev, P. H., Dorogi, M., Garab, G., Thomsen, K., Jegerschöld, C., Frigaard, N-U., Lindahl, M., & Nielsen, N. C. (2016). In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. Nature Communications, 7, [12454]. https://doi.org/10.1038/ncomms12454

Vancouver

Nielsen JT, Kulminskaya NV, Bjerring M, Linnanto JM, Rätsep M, Pedersen MØ et al. In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. Nature Communications. 2016;7. 12454. https://doi.org/10.1038/ncomms12454

Author

Nielsen, Jakob Toudahl ; Kulminskaya, Natalia V. ; Bjerring, Morten ; Linnanto, Juha M ; Rätsep, Margus ; Pedersen, Marie Østergaard ; Lambrev, Petar H. ; Dorogi, Márta ; Garab, Győző ; Thomsen, Karen ; Jegerschöld, Caroline ; Frigaard, Niels-Ulrik ; Lindahl, Martin ; Nielsen, Niels Chr. / In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. In: Nature Communications. 2016 ; Vol. 7.

Bibtex

@article{7b73b4ebf6e34a0bba0d555399173586,
title = "In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum",
abstract = "Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.",
keywords = "Journal Article",
author = "Nielsen, {Jakob Toudahl} and Kulminskaya, {Natalia V.} and Morten Bjerring and Linnanto, {Juha M} and Margus R{\"a}tsep and Pedersen, {Marie {\O}stergaard} and Lambrev, {Petar H.} and M{\'a}rta Dorogi and Gy{\H o}z{\H o} Garab and Karen Thomsen and Caroline Jegersch{\"o}ld and Niels-Ulrik Frigaard and Martin Lindahl and Nielsen, {Niels Chr.}",
year = "2016",
doi = "10.1038/ncomms12454",
language = "English",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

AU - Nielsen, Jakob Toudahl

AU - Kulminskaya, Natalia V.

AU - Bjerring, Morten

AU - Linnanto, Juha M

AU - Rätsep, Margus

AU - Pedersen, Marie Østergaard

AU - Lambrev, Petar H.

AU - Dorogi, Márta

AU - Garab, Győző

AU - Thomsen, Karen

AU - Jegerschöld, Caroline

AU - Frigaard, Niels-Ulrik

AU - Lindahl, Martin

AU - Nielsen, Niels Chr.

PY - 2016

Y1 - 2016

N2 - Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

AB - Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

KW - Journal Article

U2 - 10.1038/ncomms12454

DO - 10.1038/ncomms12454

M3 - Journal article

C2 - 27534696

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12454

ER -

ID: 164613352