In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

Research output: Contribution to conference › Conference abstract for conference › Research

Standard

In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris). / Steffensen, John Fleng.

2012.

Research output: Contribution to conference › Conference abstract for conference › Research

Harvard

Steffensen, JF 2012, 'In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)'.

APA

Steffensen, J. F. (2012). In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris).

Vancouver

Steffensen JF. In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris). 2012.

Author

Steffensen, John Fleng. / In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris).

Bibtex

@conference{340ae9c8ed044dc9b76a159ca3d6af9d,
title = "In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)",
abstract = "Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: mortenbosvendsen@gmail.com",
author = "Steffensen, {John Fleng}",
year = "2012",
language = "English",

}

RIS

TY - ABST

T1 - In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

AU - Steffensen, John Fleng

PY - 2012

Y1 - 2012

N2 - Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: mortenbosvendsen@gmail.com

AB - Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: mortenbosvendsen@gmail.com

M3 - Conference abstract for conference

ER -

ID: 45042367