Intracellular pH in rat pancreatic ducts.
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Intracellular pH in rat pancreatic ducts. / Novak, I; Hug, M; Greger, R.
In: Comparative biochemistry and physiology. Part A, Physiology, Vol. 118, No. 2, 1997, p. 409-11.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Intracellular pH in rat pancreatic ducts.
AU - Novak, I
AU - Hug, M
AU - Greger, R
N1 - Keywords: Animals; Bicarbonates; Chlorides; Hydrogen-Ion Concentration; Pancreatic Ducts; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter
PY - 1997
Y1 - 1997
N2 - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.
AB - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.
M3 - Journal article
C2 - 9366082
VL - 118
SP - 409
EP - 411
JO - Comparative Biochemistry and Physiology - A Physiology
JF - Comparative Biochemistry and Physiology - A Physiology
SN - 1096-4940
IS - 2
ER -
ID: 8570012