Intracellular pH in rat pancreatic ducts.

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Intracellular pH in rat pancreatic ducts. / Novak, I; Hug, M; Greger, R.

In: Comparative biochemistry and physiology. Part A, Physiology, Vol. 118, No. 2, 1997, p. 409-11.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Novak, I, Hug, M & Greger, R 1997, 'Intracellular pH in rat pancreatic ducts.', Comparative biochemistry and physiology. Part A, Physiology, vol. 118, no. 2, pp. 409-11.

APA

Novak, I., Hug, M., & Greger, R. (1997). Intracellular pH in rat pancreatic ducts. Comparative biochemistry and physiology. Part A, Physiology, 118(2), 409-11.

Vancouver

Novak I, Hug M, Greger R. Intracellular pH in rat pancreatic ducts. Comparative biochemistry and physiology. Part A, Physiology. 1997;118(2):409-11.

Author

Novak, I ; Hug, M ; Greger, R. / Intracellular pH in rat pancreatic ducts. In: Comparative biochemistry and physiology. Part A, Physiology. 1997 ; Vol. 118, No. 2. pp. 409-11.

Bibtex

@article{f4a42360b18411ddb04f000ea68e967b,
title = "Intracellular pH in rat pancreatic ducts.",
abstract = "In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.",
author = "I Novak and M Hug and R Greger",
note = "Keywords: Animals; Bicarbonates; Chlorides; Hydrogen-Ion Concentration; Pancreatic Ducts; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter",
year = "1997",
language = "English",
volume = "118",
pages = "409--11",
journal = "Comparative Biochemistry and Physiology - A Physiology",
issn = "1096-4940",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Intracellular pH in rat pancreatic ducts.

AU - Novak, I

AU - Hug, M

AU - Greger, R

N1 - Keywords: Animals; Bicarbonates; Chlorides; Hydrogen-Ion Concentration; Pancreatic Ducts; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter

PY - 1997

Y1 - 1997

N2 - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.

AB - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.

M3 - Journal article

C2 - 9366082

VL - 118

SP - 409

EP - 411

JO - Comparative Biochemistry and Physiology - A Physiology

JF - Comparative Biochemistry and Physiology - A Physiology

SN - 1096-4940

IS - 2

ER -

ID: 8570012