TY - JOUR

T1 - Investigation of diversity of plasmids carrying the blaTEM-52 gene

AU - Bielak, Eliza Maria

AU - Bergenholtz, Rikke D

AU - Jørgensen, Mikael Skaanning

AU - Sørensen, Søren J

AU - Hansen, Lars H

AU - Hasman, Henrik

PY - 2011

Y1 - 2011

N2 - Objectives: To investigate the diversity of plasmids that carry blaTEM-52 genes among Escherichia coli andSalmonella enterica originating from animals, meat products and humans.Methods: A collection of 22 blaTEM-52-encoding plasmids was characterized by restriction fragment lengthpolymorphism (RFLP), replicon typing (by PCR or replicon sequencing), susceptibility testing, assessment ofplasmid ability to self-transfer by conjugation and typing of the genetic environment of the blaTEM-52 gene.Detected IncI1 plasmids underwent further plasmid multilocus sequence typing.Results: RFLP profiles demonstrated dissemination of blaTEM-52 in Denmark (imported meat from Germany),France, Belgium and the Netherlands from 2000 to 2006 by mainly two different plasmids, one encodingblaTEM-52b (IncX1A, 45 kb) and the other blaTEM-52c (IncI1, 80 kb). In addition, blaTEM-52b was also found to belocated on various other plasmids belonging to IncA/C and IncL/M, while blaTEM-52c was found on IncN-likeas well as on IncR plasmids. In the majority of cases (n¼21) the blaTEM-52 gene was located on a Tn3 transposon.Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transferto a plasmid-free E. coli recipient.Conclusions: The blaTEM-52 gene found in humans could have been transmitted on transferable plasmids originatingfrom animal sources. Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones.Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor blaTEM-1, the blaTEM-52 gene wasnot detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.

AB - Objectives: To investigate the diversity of plasmids that carry blaTEM-52 genes among Escherichia coli andSalmonella enterica originating from animals, meat products and humans.Methods: A collection of 22 blaTEM-52-encoding plasmids was characterized by restriction fragment lengthpolymorphism (RFLP), replicon typing (by PCR or replicon sequencing), susceptibility testing, assessment ofplasmid ability to self-transfer by conjugation and typing of the genetic environment of the blaTEM-52 gene.Detected IncI1 plasmids underwent further plasmid multilocus sequence typing.Results: RFLP profiles demonstrated dissemination of blaTEM-52 in Denmark (imported meat from Germany),France, Belgium and the Netherlands from 2000 to 2006 by mainly two different plasmids, one encodingblaTEM-52b (IncX1A, 45 kb) and the other blaTEM-52c (IncI1, 80 kb). In addition, blaTEM-52b was also found to belocated on various other plasmids belonging to IncA/C and IncL/M, while blaTEM-52c was found on IncN-likeas well as on IncR plasmids. In the majority of cases (n¼21) the blaTEM-52 gene was located on a Tn3 transposon.Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transferto a plasmid-free E. coli recipient.Conclusions: The blaTEM-52 gene found in humans could have been transmitted on transferable plasmids originatingfrom animal sources. Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones.Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor blaTEM-1, the blaTEM-52 gene wasnot detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.

U2 - 10.1093/jac/dkr331

DO - 10.1093/jac/dkr331

M3 - Journal article

C2 - 21831988

VL - 66

SP - 2465

EP - 2474

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 11

ER -