TY - JOUR

T1 - Mitochondrial DNA diversity and population structure of a forest-dependent rodent, Praomys taitae (Rodentia: Muridae) Heller 1911, in the fragmented forest patches of Taita Hills, Kenya

AU - Nyakaana, S.

AU - Tumusiime, C.

AU - Oguge, N.

AU - Siegismund, Hans Redlef

AU - Arctander, Peter

AU - Muwanika, V.

N1 - KeyWords Plus: AFROTROPICAL FOREST; STATISTICAL TESTS; GENETIC-STRUCTURE; POLYMORPHISM; HABITAT; BIRD; HITCHHIKING; EXTINCTION; DEMOGRAPHY; NEUTRALITY

PY - 2008

Y1 - 2008

N2 - The population genetic structure of the forest-dependent rodent,Praomys taitae, sampled from nine indigenous forest fragmentssampled from nine indigenous forest fragments distributed over three ranges of the Taita Hills in Kenya, was determinedusing mitochondrial DNA (mtDNA) control region sequencevariation. Thirty-three unique haplotypes were observed in a totalsample of 132 individuals, whereas the number of different haplotypesper population ranged from two to 10. An overall low nucleotidediversity of 0.9% was observed in the total sample but varied widelybetween populations (0.2-1.3%). Significant genetic differentiationwas observed in 30 of the 36 possible pair-wise comparisonsbetween populations, while a hierarchical AMOVA revealed significantgenetic subdivision between groups of populations on thethree hill ranges of Dabida, Mbololo and Kyulu (FCT = 0.404, P <0.01), among populations on each of the hill ranges (FSC = 0.112,P < 0.01) and among populations in the total sample (FST = 0.471,< 0.01) and among populations in the total sample (ST = 0.471, P < 0.001). Demographic history analyses based on pair-wise< 0.001). Demographic history analyses based on pair-wise nucleotide sequence mismatch distributions revealed that all thepopulations were in mutation-drift disequilibrium except the populationsof the Kyulu and Ronge forest fragments.

AB - The population genetic structure of the forest-dependent rodent,Praomys taitae, sampled from nine indigenous forest fragmentssampled from nine indigenous forest fragments distributed over three ranges of the Taita Hills in Kenya, was determinedusing mitochondrial DNA (mtDNA) control region sequencevariation. Thirty-three unique haplotypes were observed in a totalsample of 132 individuals, whereas the number of different haplotypesper population ranged from two to 10. An overall low nucleotidediversity of 0.9% was observed in the total sample but varied widelybetween populations (0.2-1.3%). Significant genetic differentiationwas observed in 30 of the 36 possible pair-wise comparisonsbetween populations, while a hierarchical AMOVA revealed significantgenetic subdivision between groups of populations on thethree hill ranges of Dabida, Mbololo and Kyulu (FCT = 0.404, P <0.01), among populations on each of the hill ranges (FSC = 0.112,P < 0.01) and among populations in the total sample (FST = 0.471,< 0.01) and among populations in the total sample (ST = 0.471, P < 0.001). Demographic history analyses based on pair-wise< 0.001). Demographic history analyses based on pair-wise nucleotide sequence mismatch distributions revealed that all thepopulations were in mutation-drift disequilibrium except the populationsof the Kyulu and Ronge forest fragments.

M3 - Journal article

VL - 104

SP - 499

EP - 504

JO - South African Journal of Science

JF - South African Journal of Science

SN - 0038-2353

IS - 11/12

ER -