Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2.

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Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2. / Rosenkilde, Carina; Cazzamali, Giuseppe; Williamson, Michael; Hauser, Frank; Søndergaard, Leif; DeLotto, Robert; Grimmelikhuijzen, Cornelis J P.

In: Biochemical and Biophysical Research Communications, Vol. 309, No. 2, 2003, p. 485-94.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rosenkilde, C, Cazzamali, G, Williamson, M, Hauser, F, Søndergaard, L, DeLotto, R & Grimmelikhuijzen, CJP 2003, 'Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2.', Biochemical and Biophysical Research Communications, vol. 309, no. 2, pp. 485-94. https://doi.org/10.1016/j.bbrc.2003.08.022

APA

Rosenkilde, C., Cazzamali, G., Williamson, M., Hauser, F., Søndergaard, L., DeLotto, R., & Grimmelikhuijzen, C. J. P. (2003). Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2. Biochemical and Biophysical Research Communications, 309(2), 485-94. https://doi.org/10.1016/j.bbrc.2003.08.022

Vancouver

Rosenkilde C, Cazzamali G, Williamson M, Hauser F, Søndergaard L, DeLotto R et al. Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2. Biochemical and Biophysical Research Communications. 2003;309(2):485-94. https://doi.org/10.1016/j.bbrc.2003.08.022

Author

Rosenkilde, Carina ; Cazzamali, Giuseppe ; Williamson, Michael ; Hauser, Frank ; Søndergaard, Leif ; DeLotto, Robert ; Grimmelikhuijzen, Cornelis J P. / Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 309, No. 2. pp. 485-94.

Bibtex

@article{e6393220ec2711dcbee902004c4f4f50,
title = "Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2.",
abstract = "The database of the Drosophila Genome Project contains the sequences of two genes, CG8784 and CG8795, predicted to code for two structurally related G protein-coupled receptors. We have cloned these genes and expressed their coding parts in Chinese hamster ovary cells. We found that both receptors can be activated by low concentrations of the Drosophila neuropeptide pyrokinin-2 (CG8784, EC(50) for pyrokinin-2, 1x10(-9)M; CG8795, EC(50) for pyrokinin-2, 5 x 10(-10)M). The precise role of Drosophila pyrokinin-2 (SVPFKPRLamide) in Drosophila is unknown, but in other insects, pyrokinins have diverse myotropic actions and are also initiating sex pheromone biosynthesis and embryonic diapause. Gene silencing, using the RNA-mediated interference technique, showed that CG8784 gene silencing caused lethality in embryos, whereas CG8795 gene silencing resulted in strongly reduced viability for both embryos and first instar larvae. In addition to the two Drosophila receptors, we also identified two probable pyrokinin receptors in the genomic database from the malaria mosquito Anopheles gambiae. The two Drosophila pyrokinin receptors are, to our knowledge, the first invertebrate pyrokinin receptors to be identified.",
author = "Carina Rosenkilde and Giuseppe Cazzamali and Michael Williamson and Frank Hauser and Leif S{\o}ndergaard and Robert DeLotto and Grimmelikhuijzen, {Cornelis J P}",
note = "Keywords: Amino Acid Sequence; Animals; CHO Cells; Cloning, Molecular; Cricetinae; Drosophila; GTP-Binding Proteins; Gene Expression Regulation; Gene Silencing; Molecular Sequence Data; Neuropeptides; Sequence Alignment; Sequence Analysis, Protein; Transfection",
year = "2003",
doi = "10.1016/j.bbrc.2003.08.022",
language = "English",
volume = "309",
pages = "485--94",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Molecular cloning, functional expression, and gene silencing of two Drosophila receptors for the Drosophila neuropeptide pyrokinin-2.

AU - Rosenkilde, Carina

AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Hauser, Frank

AU - Søndergaard, Leif

AU - DeLotto, Robert

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; CHO Cells; Cloning, Molecular; Cricetinae; Drosophila; GTP-Binding Proteins; Gene Expression Regulation; Gene Silencing; Molecular Sequence Data; Neuropeptides; Sequence Alignment; Sequence Analysis, Protein; Transfection

PY - 2003

Y1 - 2003

N2 - The database of the Drosophila Genome Project contains the sequences of two genes, CG8784 and CG8795, predicted to code for two structurally related G protein-coupled receptors. We have cloned these genes and expressed their coding parts in Chinese hamster ovary cells. We found that both receptors can be activated by low concentrations of the Drosophila neuropeptide pyrokinin-2 (CG8784, EC(50) for pyrokinin-2, 1x10(-9)M; CG8795, EC(50) for pyrokinin-2, 5 x 10(-10)M). The precise role of Drosophila pyrokinin-2 (SVPFKPRLamide) in Drosophila is unknown, but in other insects, pyrokinins have diverse myotropic actions and are also initiating sex pheromone biosynthesis and embryonic diapause. Gene silencing, using the RNA-mediated interference technique, showed that CG8784 gene silencing caused lethality in embryos, whereas CG8795 gene silencing resulted in strongly reduced viability for both embryos and first instar larvae. In addition to the two Drosophila receptors, we also identified two probable pyrokinin receptors in the genomic database from the malaria mosquito Anopheles gambiae. The two Drosophila pyrokinin receptors are, to our knowledge, the first invertebrate pyrokinin receptors to be identified.

AB - The database of the Drosophila Genome Project contains the sequences of two genes, CG8784 and CG8795, predicted to code for two structurally related G protein-coupled receptors. We have cloned these genes and expressed their coding parts in Chinese hamster ovary cells. We found that both receptors can be activated by low concentrations of the Drosophila neuropeptide pyrokinin-2 (CG8784, EC(50) for pyrokinin-2, 1x10(-9)M; CG8795, EC(50) for pyrokinin-2, 5 x 10(-10)M). The precise role of Drosophila pyrokinin-2 (SVPFKPRLamide) in Drosophila is unknown, but in other insects, pyrokinins have diverse myotropic actions and are also initiating sex pheromone biosynthesis and embryonic diapause. Gene silencing, using the RNA-mediated interference technique, showed that CG8784 gene silencing caused lethality in embryos, whereas CG8795 gene silencing resulted in strongly reduced viability for both embryos and first instar larvae. In addition to the two Drosophila receptors, we also identified two probable pyrokinin receptors in the genomic database from the malaria mosquito Anopheles gambiae. The two Drosophila pyrokinin receptors are, to our knowledge, the first invertebrate pyrokinin receptors to be identified.

U2 - 10.1016/j.bbrc.2003.08.022

DO - 10.1016/j.bbrc.2003.08.022

M3 - Journal article

C2 - 12951076

VL - 309

SP - 485

EP - 494

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 3045850