Molecular identification of the first insect ecdysis triggering hormone receptors.

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Molecular identification of the first insect ecdysis triggering hormone receptors. / Iversen, Annette; Cazzamali, Giuseppe; Williamson, Michael; Hauser, Frank; Grimmelikhuijzen, Cornelis J P.

In: Biochemical and Biophysical Research Communications, Vol. 299, No. 5, 2002, p. 924-31.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Iversen, A, Cazzamali, G, Williamson, M, Hauser, F & Grimmelikhuijzen, CJP 2002, 'Molecular identification of the first insect ecdysis triggering hormone receptors.', Biochemical and Biophysical Research Communications, vol. 299, no. 5, pp. 924-31. https://doi.org/10.1016/S0006-291X(02)02798-5

APA

Iversen, A., Cazzamali, G., Williamson, M., Hauser, F., & Grimmelikhuijzen, C. J. P. (2002). Molecular identification of the first insect ecdysis triggering hormone receptors. Biochemical and Biophysical Research Communications, 299(5), 924-31. https://doi.org/10.1016/S0006-291X(02)02798-5

Vancouver

Iversen A, Cazzamali G, Williamson M, Hauser F, Grimmelikhuijzen CJP. Molecular identification of the first insect ecdysis triggering hormone receptors. Biochemical and Biophysical Research Communications. 2002;299(5):924-31. https://doi.org/10.1016/S0006-291X(02)02798-5

Author

Iversen, Annette ; Cazzamali, Giuseppe ; Williamson, Michael ; Hauser, Frank ; Grimmelikhuijzen, Cornelis J P. / Molecular identification of the first insect ecdysis triggering hormone receptors. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 299, No. 5. pp. 924-31.

Bibtex

@article{fc922c70ec2711dcbee902004c4f4f50,
title = "Molecular identification of the first insect ecdysis triggering hormone receptors.",
abstract = "The Drosophila Genome Project website (www.flybase.org) contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.",
author = "Annette Iversen and Giuseppe Cazzamali and Michael Williamson and Frank Hauser and Grimmelikhuijzen, {Cornelis J P}",
note = "Keywords: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; Drosophila; Drosophila Proteins; Insect Hormones; Molecular Sequence Data; Molting; RNA, Messenger; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Receptors, Neuropeptide; Sequence Homology, Amino Acid",
year = "2002",
doi = "10.1016/S0006-291X(02)02798-5",
language = "English",
volume = "299",
pages = "924--31",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Molecular identification of the first insect ecdysis triggering hormone receptors.

AU - Iversen, Annette

AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Hauser, Frank

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; Drosophila; Drosophila Proteins; Insect Hormones; Molecular Sequence Data; Molting; RNA, Messenger; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Receptors, Neuropeptide; Sequence Homology, Amino Acid

PY - 2002

Y1 - 2002

N2 - The Drosophila Genome Project website (www.flybase.org) contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.

AB - The Drosophila Genome Project website (www.flybase.org) contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.

U2 - 10.1016/S0006-291X(02)02798-5

DO - 10.1016/S0006-291X(02)02798-5

M3 - Journal article

C2 - 12470668

VL - 299

SP - 924

EP - 931

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 5

ER -

ID: 3045915