Molecular identification of the first insect proctolin receptor.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Molecular identification of the first insect proctolin receptor. / Egerod, Kristoffer; Reynisson, Eyjólfur; Hauser, Frank; Williamson, Michael; Cazzamali, Giuseppe; Grimmelikhuijzen, Cornelis J P.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 2, 2003, p. 437-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Egerod, K, Reynisson, E, Hauser, F, Williamson, M, Cazzamali, G & Grimmelikhuijzen, CJP 2003, 'Molecular identification of the first insect proctolin receptor.', Biochemical and Biophysical Research Communications, vol. 306, no. 2, pp. 437-42. https://doi.org/10.1016/S0006-291X(03)00997-5

APA

Egerod, K., Reynisson, E., Hauser, F., Williamson, M., Cazzamali, G., & Grimmelikhuijzen, C. J. P. (2003). Molecular identification of the first insect proctolin receptor. Biochemical and Biophysical Research Communications, 306(2), 437-42. https://doi.org/10.1016/S0006-291X(03)00997-5

Vancouver

Egerod K, Reynisson E, Hauser F, Williamson M, Cazzamali G, Grimmelikhuijzen CJP. Molecular identification of the first insect proctolin receptor. Biochemical and Biophysical Research Communications. 2003;306(2):437-42. https://doi.org/10.1016/S0006-291X(03)00997-5

Author

Egerod, Kristoffer ; Reynisson, Eyjólfur ; Hauser, Frank ; Williamson, Michael ; Cazzamali, Giuseppe ; Grimmelikhuijzen, Cornelis J P. / Molecular identification of the first insect proctolin receptor. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 2. pp. 437-42.

Bibtex

@article{f335a0d0ec2711dcbee902004c4f4f50,
title = "Molecular identification of the first insect proctolin receptor.",
abstract = "The website of the Drosophila Genome Project (www.flybase.org) contains the sequence of an annotated gene CG6986, which is predicted to code for a G protein-coupled receptor. We cloned the cDNA of this gene and expressed it in Chinese hamster ovary cells. Screening of a neuropeptide library revealed that the expressed receptor was specific for the neuropeptide proctolin (EC(50), 6x10(-10)M). Proctolin (RYLPT) was the first invertebrate neuropeptide to be fully sequenced (already in 1975) and occurs with identical structure in both crustaceans and insects, where it has myo- and neurostimulatory actions. Northern blots showed that the Drosophila proctolin receptor was only weakly expressed in embryos, larvae, pupae, and in the thoraces and abdomina of adult flies, but strongly in the heads of adult animals. The Drosophila receptor reported here is the first invertebrate proctolin receptor to be identified.",
author = "Kristoffer Egerod and Eyj{\'o}lfur Reynisson and Frank Hauser and Michael Williamson and Giuseppe Cazzamali and Grimmelikhuijzen, {Cornelis J P}",
note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; CHO Cells; Cell Line; Cloning, Molecular; Cricetinae; DNA, Complementary; Dose-Response Relationship, Drug; Drosophila; Drosophila Proteins; Exons; GTP-Binding Proteins; Gene Library; Genome; Introns; Ligands; Models, Genetic; Molecular Sequence Data; Neuropeptides; RNA; Receptors, Cell Surface; Receptors, Peptide; Time Factors",
year = "2003",
doi = "10.1016/S0006-291X(03)00997-5",
language = "English",
volume = "306",
pages = "437--42",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Molecular identification of the first insect proctolin receptor.

AU - Egerod, Kristoffer

AU - Reynisson, Eyjólfur

AU - Hauser, Frank

AU - Williamson, Michael

AU - Cazzamali, Giuseppe

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; CHO Cells; Cell Line; Cloning, Molecular; Cricetinae; DNA, Complementary; Dose-Response Relationship, Drug; Drosophila; Drosophila Proteins; Exons; GTP-Binding Proteins; Gene Library; Genome; Introns; Ligands; Models, Genetic; Molecular Sequence Data; Neuropeptides; RNA; Receptors, Cell Surface; Receptors, Peptide; Time Factors

PY - 2003

Y1 - 2003

N2 - The website of the Drosophila Genome Project (www.flybase.org) contains the sequence of an annotated gene CG6986, which is predicted to code for a G protein-coupled receptor. We cloned the cDNA of this gene and expressed it in Chinese hamster ovary cells. Screening of a neuropeptide library revealed that the expressed receptor was specific for the neuropeptide proctolin (EC(50), 6x10(-10)M). Proctolin (RYLPT) was the first invertebrate neuropeptide to be fully sequenced (already in 1975) and occurs with identical structure in both crustaceans and insects, where it has myo- and neurostimulatory actions. Northern blots showed that the Drosophila proctolin receptor was only weakly expressed in embryos, larvae, pupae, and in the thoraces and abdomina of adult flies, but strongly in the heads of adult animals. The Drosophila receptor reported here is the first invertebrate proctolin receptor to be identified.

AB - The website of the Drosophila Genome Project (www.flybase.org) contains the sequence of an annotated gene CG6986, which is predicted to code for a G protein-coupled receptor. We cloned the cDNA of this gene and expressed it in Chinese hamster ovary cells. Screening of a neuropeptide library revealed that the expressed receptor was specific for the neuropeptide proctolin (EC(50), 6x10(-10)M). Proctolin (RYLPT) was the first invertebrate neuropeptide to be fully sequenced (already in 1975) and occurs with identical structure in both crustaceans and insects, where it has myo- and neurostimulatory actions. Northern blots showed that the Drosophila proctolin receptor was only weakly expressed in embryos, larvae, pupae, and in the thoraces and abdomina of adult flies, but strongly in the heads of adult animals. The Drosophila receptor reported here is the first invertebrate proctolin receptor to be identified.

U2 - 10.1016/S0006-291X(03)00997-5

DO - 10.1016/S0006-291X(03)00997-5

M3 - Journal article

C2 - 12804582

VL - 306

SP - 437

EP - 442

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 3045885