Molecular identification of the first SIFamide receptor.

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Molecular identification of the first SIFamide receptor. / Jørgensen, Lars M; Hauser, Frank; Cazzamali, Giuseppe; Williamson, Michael; Grimmelikhuijzen, Cornelis J P.

In: Biochemical and Biophysical Research Communications, Vol. 340, No. 2, 2006, p. 696-701.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, LM, Hauser, F, Cazzamali, G, Williamson, M & Grimmelikhuijzen, CJP 2006, 'Molecular identification of the first SIFamide receptor.', Biochemical and Biophysical Research Communications, vol. 340, no. 2, pp. 696-701. https://doi.org/10.1016/j.bbrc.2005.12.062

APA

Jørgensen, L. M., Hauser, F., Cazzamali, G., Williamson, M., & Grimmelikhuijzen, C. J. P. (2006). Molecular identification of the first SIFamide receptor. Biochemical and Biophysical Research Communications, 340(2), 696-701. https://doi.org/10.1016/j.bbrc.2005.12.062

Vancouver

Jørgensen LM, Hauser F, Cazzamali G, Williamson M, Grimmelikhuijzen CJP. Molecular identification of the first SIFamide receptor. Biochemical and Biophysical Research Communications. 2006;340(2):696-701. https://doi.org/10.1016/j.bbrc.2005.12.062

Author

Jørgensen, Lars M ; Hauser, Frank ; Cazzamali, Giuseppe ; Williamson, Michael ; Grimmelikhuijzen, Cornelis J P. / Molecular identification of the first SIFamide receptor. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 340, No. 2. pp. 696-701.

Bibtex

@article{cc338380ec2711dcbee902004c4f4f50,
title = "Molecular identification of the first SIFamide receptor.",
abstract = "SIFamide is the short name and also the C terminus of the Drosophila neuropeptide AYRKPPFNGSIFamide. SIFamide has been isolated or predicted from various insects and crustaceans, and appears to be extremely well conserved among these arthropods. However, the function of this neuropeptide is still enigmatic. Here, we have identified the Drosophila gene (CG10823) coding for the SIFamide receptor. When expressed in Chinese hamster ovary cells, the receptor is only activated by Drosophila SIFamide (EC(50), 2x10(-8)M) and not by a library of 32 other insect neuropeptides and eight biogenic amines. Database searches revealed SIFamide receptor orthologues in the genomes from the malaria mosquito Anopheles gambiae, the silkworm Bombyx mori, the red flour beetle Tribolium castaneum, and the honey bee Apis mellifera. An alignment of the five insect SIFamide or SIFamide-like receptors showed, again, an impressive sequence conservation (67-77% amino acid sequence identities between the seven-transmembrane areas; 82-87% sequence similarities). The identification of well-conserved SIFamide receptor orthologues in all other insects with a sequenced genome, suggests that the SIFamide/receptor couple must have an essential function in arthropods. This paper is the first report on the identification of a SIFamide receptor.",
author = "J{\o}rgensen, {Lars M} and Frank Hauser and Giuseppe Cazzamali and Michael Williamson and Grimmelikhuijzen, {Cornelis J P}",
note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; Cricetulus; DNA, Complementary; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Neuropeptides; Receptors, G-Protein-Coupled; Receptors, Neuropeptide",
year = "2006",
doi = "10.1016/j.bbrc.2005.12.062",
language = "English",
volume = "340",
pages = "696--701",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Molecular identification of the first SIFamide receptor.

AU - Jørgensen, Lars M

AU - Hauser, Frank

AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; Cricetulus; DNA, Complementary; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Neuropeptides; Receptors, G-Protein-Coupled; Receptors, Neuropeptide

PY - 2006

Y1 - 2006

N2 - SIFamide is the short name and also the C terminus of the Drosophila neuropeptide AYRKPPFNGSIFamide. SIFamide has been isolated or predicted from various insects and crustaceans, and appears to be extremely well conserved among these arthropods. However, the function of this neuropeptide is still enigmatic. Here, we have identified the Drosophila gene (CG10823) coding for the SIFamide receptor. When expressed in Chinese hamster ovary cells, the receptor is only activated by Drosophila SIFamide (EC(50), 2x10(-8)M) and not by a library of 32 other insect neuropeptides and eight biogenic amines. Database searches revealed SIFamide receptor orthologues in the genomes from the malaria mosquito Anopheles gambiae, the silkworm Bombyx mori, the red flour beetle Tribolium castaneum, and the honey bee Apis mellifera. An alignment of the five insect SIFamide or SIFamide-like receptors showed, again, an impressive sequence conservation (67-77% amino acid sequence identities between the seven-transmembrane areas; 82-87% sequence similarities). The identification of well-conserved SIFamide receptor orthologues in all other insects with a sequenced genome, suggests that the SIFamide/receptor couple must have an essential function in arthropods. This paper is the first report on the identification of a SIFamide receptor.

AB - SIFamide is the short name and also the C terminus of the Drosophila neuropeptide AYRKPPFNGSIFamide. SIFamide has been isolated or predicted from various insects and crustaceans, and appears to be extremely well conserved among these arthropods. However, the function of this neuropeptide is still enigmatic. Here, we have identified the Drosophila gene (CG10823) coding for the SIFamide receptor. When expressed in Chinese hamster ovary cells, the receptor is only activated by Drosophila SIFamide (EC(50), 2x10(-8)M) and not by a library of 32 other insect neuropeptides and eight biogenic amines. Database searches revealed SIFamide receptor orthologues in the genomes from the malaria mosquito Anopheles gambiae, the silkworm Bombyx mori, the red flour beetle Tribolium castaneum, and the honey bee Apis mellifera. An alignment of the five insect SIFamide or SIFamide-like receptors showed, again, an impressive sequence conservation (67-77% amino acid sequence identities between the seven-transmembrane areas; 82-87% sequence similarities). The identification of well-conserved SIFamide receptor orthologues in all other insects with a sequenced genome, suggests that the SIFamide/receptor couple must have an essential function in arthropods. This paper is the first report on the identification of a SIFamide receptor.

U2 - 10.1016/j.bbrc.2005.12.062

DO - 10.1016/j.bbrc.2005.12.062

M3 - Journal article

C2 - 16378592

VL - 340

SP - 696

EP - 701

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 3045779