Nucleosomes are well positioned in exons and carry characteristic histone modifications

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Nucleosomes are well positioned in exons and carry characteristic histone modifications. / Andersson, Robin; Enroth, Stefan; Rada-Iglesias, Alvaro; Wadelius, Claes; Komorowski, Jan.

In: Genome Research, Vol. 19, No. 10, 10.2009, p. 1732-41.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersson, R, Enroth, S, Rada-Iglesias, A, Wadelius, C & Komorowski, J 2009, 'Nucleosomes are well positioned in exons and carry characteristic histone modifications', Genome Research, vol. 19, no. 10, pp. 1732-41. https://doi.org/10.1101/gr.092353.109

APA

Andersson, R., Enroth, S., Rada-Iglesias, A., Wadelius, C., & Komorowski, J. (2009). Nucleosomes are well positioned in exons and carry characteristic histone modifications. Genome Research, 19(10), 1732-41. https://doi.org/10.1101/gr.092353.109

Vancouver

Andersson R, Enroth S, Rada-Iglesias A, Wadelius C, Komorowski J. Nucleosomes are well positioned in exons and carry characteristic histone modifications. Genome Research. 2009 Oct;19(10):1732-41. https://doi.org/10.1101/gr.092353.109

Author

Andersson, Robin ; Enroth, Stefan ; Rada-Iglesias, Alvaro ; Wadelius, Claes ; Komorowski, Jan. / Nucleosomes are well positioned in exons and carry characteristic histone modifications. In: Genome Research. 2009 ; Vol. 19, No. 10. pp. 1732-41.

Bibtex

@article{ddd7acd058f549dc9e177171f5482372,
title = "Nucleosomes are well positioned in exons and carry characteristic histone modifications",
abstract = "The genomes of higher organisms are packaged in nucleosomes with functional histone modifications. Until now, genome-wide nucleosome and histone modification studies have focused on transcription start sites (TSSs) where nucleosomes in RNA polymerase II (RNAPII) occupied genes are well positioned and have histone modifications that are characteristic of expression status. Using public data, we here show that there is a higher nucleosome-positioning signal in internal human exons and that this positioning is independent of expression. We observed a similarly strong nucleosome-positioning signal in internal exons of Caenorhabditis elegans. Among the 38 histone modifications analyzed in man, H3K36me3, H3K79me1, H2BK5me1, H3K27me1, H3K27me2, and H3K27me3 had evidently higher signals in internal exons than in the following introns and were clearly related to exon expression. These observations are suggestive of roles in splicing. Thus, exons are not only characterized by their coding capacity, but also by their nucleosome organization, which seems evolutionarily conserved since it is present in both primates and nematodes.",
keywords = "Alternative Splicing, Animals, Caenorhabditis, Cells, Cultured, Exons, Gene Expression Regulation, Gene Order, Histone-Lysine N-Methyltransferase, Histones, Humans, Mice, Nucleosomes, Primates, Protein Processing, Post-Translational, Transcription, Genetic",
author = "Robin Andersson and Stefan Enroth and Alvaro Rada-Iglesias and Claes Wadelius and Jan Komorowski",
year = "2009",
month = oct,
doi = "10.1101/gr.092353.109",
language = "English",
volume = "19",
pages = "1732--41",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "10",

}

RIS

TY - JOUR

T1 - Nucleosomes are well positioned in exons and carry characteristic histone modifications

AU - Andersson, Robin

AU - Enroth, Stefan

AU - Rada-Iglesias, Alvaro

AU - Wadelius, Claes

AU - Komorowski, Jan

PY - 2009/10

Y1 - 2009/10

N2 - The genomes of higher organisms are packaged in nucleosomes with functional histone modifications. Until now, genome-wide nucleosome and histone modification studies have focused on transcription start sites (TSSs) where nucleosomes in RNA polymerase II (RNAPII) occupied genes are well positioned and have histone modifications that are characteristic of expression status. Using public data, we here show that there is a higher nucleosome-positioning signal in internal human exons and that this positioning is independent of expression. We observed a similarly strong nucleosome-positioning signal in internal exons of Caenorhabditis elegans. Among the 38 histone modifications analyzed in man, H3K36me3, H3K79me1, H2BK5me1, H3K27me1, H3K27me2, and H3K27me3 had evidently higher signals in internal exons than in the following introns and were clearly related to exon expression. These observations are suggestive of roles in splicing. Thus, exons are not only characterized by their coding capacity, but also by their nucleosome organization, which seems evolutionarily conserved since it is present in both primates and nematodes.

AB - The genomes of higher organisms are packaged in nucleosomes with functional histone modifications. Until now, genome-wide nucleosome and histone modification studies have focused on transcription start sites (TSSs) where nucleosomes in RNA polymerase II (RNAPII) occupied genes are well positioned and have histone modifications that are characteristic of expression status. Using public data, we here show that there is a higher nucleosome-positioning signal in internal human exons and that this positioning is independent of expression. We observed a similarly strong nucleosome-positioning signal in internal exons of Caenorhabditis elegans. Among the 38 histone modifications analyzed in man, H3K36me3, H3K79me1, H2BK5me1, H3K27me1, H3K27me2, and H3K27me3 had evidently higher signals in internal exons than in the following introns and were clearly related to exon expression. These observations are suggestive of roles in splicing. Thus, exons are not only characterized by their coding capacity, but also by their nucleosome organization, which seems evolutionarily conserved since it is present in both primates and nematodes.

KW - Alternative Splicing

KW - Animals

KW - Caenorhabditis

KW - Cells, Cultured

KW - Exons

KW - Gene Expression Regulation

KW - Gene Order

KW - Histone-Lysine N-Methyltransferase

KW - Histones

KW - Humans

KW - Mice

KW - Nucleosomes

KW - Primates

KW - Protein Processing, Post-Translational

KW - Transcription, Genetic

U2 - 10.1101/gr.092353.109

DO - 10.1101/gr.092353.109

M3 - Journal article

C2 - 19687145

VL - 19

SP - 1732

EP - 1741

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 10

ER -

ID: 106775649