Oral administration of helminth fluid modulates distinct tuft cell and immune-metabolic cues linked to reduced body fat
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Oral administration of helminth fluid modulates distinct tuft cell and immune-metabolic cues linked to reduced body fat. / Andersen, Daniel; Moll, Janne Marie; Arora, Pankaj; Danneskiold-Samsøe, Niels Banhos; Sonne, Si Brask; Workman, Christopher Thomas; Williams, Andrew Richard; Kristiansen, Karsten; Brix, Susanne.
In: Parasite Immunology, Vol. 45, No. 7, e12998, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Oral administration of helminth fluid modulates distinct tuft cell and immune-metabolic cues linked to reduced body fat
AU - Andersen, Daniel
AU - Moll, Janne Marie
AU - Arora, Pankaj
AU - Danneskiold-Samsøe, Niels Banhos
AU - Sonne, Si Brask
AU - Workman, Christopher Thomas
AU - Williams, Andrew Richard
AU - Kristiansen, Karsten
AU - Brix, Susanne
N1 - Publisher Copyright: © 2023 The Authors. Parasite Immunology published by John Wiley & Sons Ltd.
PY - 2023
Y1 - 2023
N2 - Intestinal tuft cells have been shown to induce type 2 immune responses during viable parasite infections, but whether oral supplementation with a parasitic exudate is able to promote type 2 immune responses that have been shown to positively regulate obesogenic metabolic processes is yet unresolved. High-fat fed mice were gavaged with pseudocoelomic fluid (PCF) derived from the helminth Ascaris suum or saline thrice a week during weeks 5–9, followed by examination of intestinal tuft cell activity, immune, and metabolic parameters. Helminth PCF upregulated expression of distinct genes in small intestinal tuft cells, including genes involved in regulation of RUNX1 and organic cation transporters. Helminth PCF also enhanced levels of innate lymphoid cells in the ileum, and eosinophils in epididymal white adipose tissue (eWAT). Network analyses revealed two distinct immunometabolic cues affected by oral helminth PCF in high-fat fed mice: one coupling the small intestinal tuft cell responses to the fat-to-lean mass ratio and a second coupling eosinophils in eWAT to general regulation of body fat mass. Our findings point to specific mechanisms by which oral supplementation with helminth PCF may translate into systems-wide effects linking to reduced body and fat mass gain in mice during high-fat feeding.
AB - Intestinal tuft cells have been shown to induce type 2 immune responses during viable parasite infections, but whether oral supplementation with a parasitic exudate is able to promote type 2 immune responses that have been shown to positively regulate obesogenic metabolic processes is yet unresolved. High-fat fed mice were gavaged with pseudocoelomic fluid (PCF) derived from the helminth Ascaris suum or saline thrice a week during weeks 5–9, followed by examination of intestinal tuft cell activity, immune, and metabolic parameters. Helminth PCF upregulated expression of distinct genes in small intestinal tuft cells, including genes involved in regulation of RUNX1 and organic cation transporters. Helminth PCF also enhanced levels of innate lymphoid cells in the ileum, and eosinophils in epididymal white adipose tissue (eWAT). Network analyses revealed two distinct immunometabolic cues affected by oral helminth PCF in high-fat fed mice: one coupling the small intestinal tuft cell responses to the fat-to-lean mass ratio and a second coupling eosinophils in eWAT to general regulation of body fat mass. Our findings point to specific mechanisms by which oral supplementation with helminth PCF may translate into systems-wide effects linking to reduced body and fat mass gain in mice during high-fat feeding.
KW - energy metabolism
KW - helminth
KW - immune responses
KW - systems biology
KW - tuft cells
U2 - 10.1111/pim.12998
DO - 10.1111/pim.12998
M3 - Journal article
C2 - 37282739
AN - SCOPUS:85161513096
VL - 45
JO - Parasite Immunology
JF - Parasite Immunology
SN - 0141-9838
IS - 7
M1 - e12998
ER -
ID: 357054708