Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling. / Hansen, Jacob B.; Zhang, Hongbin; Rasmussen, Thomas H.; Petersen, Rasmus K.; Flindt, Esben N.; Kristiansen, Karsten.

In: Journal of Biological Chemistry, Vol. 276, No. 5, 2001, p. 3175-3182.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, JB, Zhang, H, Rasmussen, TH, Petersen, RK, Flindt, EN & Kristiansen, K 2001, 'Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling', Journal of Biological Chemistry, vol. 276, no. 5, pp. 3175-3182. https://doi.org/10.1074/jbc.M005567200

APA

Hansen, J. B., Zhang, H., Rasmussen, T. H., Petersen, R. K., Flindt, E. N., & Kristiansen, K. (2001). Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling. Journal of Biological Chemistry, 276(5), 3175-3182. https://doi.org/10.1074/jbc.M005567200

Vancouver

Hansen JB, Zhang H, Rasmussen TH, Petersen RK, Flindt EN, Kristiansen K. Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling. Journal of Biological Chemistry. 2001;276(5):3175-3182. https://doi.org/10.1074/jbc.M005567200

Author

Hansen, Jacob B. ; Zhang, Hongbin ; Rasmussen, Thomas H. ; Petersen, Rasmus K. ; Flindt, Esben N. ; Kristiansen, Karsten. / Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 5. pp. 3175-3182.

Bibtex

@article{e4305650a81b11debc73000ea68e967b,
title = "Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling",
abstract = "The peroxisome proliferator-activated receptor ¿ (PPAR¿) is a key regulator of terminal adipocyte differentiation. PPARd is expressed in preadipocytes, but the importance of this PPAR subtype in adipogenesis has been a matter of debate. Here we present a critical evaluation of the role of PPARd in adipocyte differentiation. We demonstrate that treatment of NIH-3T3 fibroblasts overexpressing PPARd with standard adipogenic inducers led to induction of PPAR¿2 expression and terminal adipocyte differentiation in a manner that was strictly dependent on simultaneous administration of a PPARd ligand and methylisobutylxanthine (MIX) or other cAMP elevating agents. We further show that ligands and MIX synergistically stimulated PPARd-mediated transactivation. In 3T3-L1 preadipocytes, simultaneous administration of a PPARd-selective ligand and MIX significantly enhanced the early expression of PPAR¿ and ALBP/aP2, but only modestly promoted terminal differentiation as determined by lipid accumulation. Finally, we provide evidence that synergistic activation of PPARd promotes mitotic clonal expansion in 3T3-L1 cells with or without forced expression of PPARd. In conclusion, our results suggest that PPARd may play a role in the proliferation of adipocyte precursor cells, whereas activation of endogenous PPARd in 3T3-L1 cells appears to have only minor impact on the processes leading to terminal adipocyte differentiation.",
author = "Hansen, {Jacob B.} and Hongbin Zhang and Rasmussen, {Thomas H.} and Petersen, {Rasmus K.} and Flindt, {Esben N.} and Karsten Kristiansen",
year = "2001",
doi = "10.1074/jbc.M005567200",
language = "English",
volume = "276",
pages = "3175--3182",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Peroxisome Proliferator-activated Receptor d (PPARd)-mediated Regulation of Preadipocyte Proliferation and Gene Expression Is Dependent on cAMP Signaling

AU - Hansen, Jacob B.

AU - Zhang, Hongbin

AU - Rasmussen, Thomas H.

AU - Petersen, Rasmus K.

AU - Flindt, Esben N.

AU - Kristiansen, Karsten

PY - 2001

Y1 - 2001

N2 - The peroxisome proliferator-activated receptor ¿ (PPAR¿) is a key regulator of terminal adipocyte differentiation. PPARd is expressed in preadipocytes, but the importance of this PPAR subtype in adipogenesis has been a matter of debate. Here we present a critical evaluation of the role of PPARd in adipocyte differentiation. We demonstrate that treatment of NIH-3T3 fibroblasts overexpressing PPARd with standard adipogenic inducers led to induction of PPAR¿2 expression and terminal adipocyte differentiation in a manner that was strictly dependent on simultaneous administration of a PPARd ligand and methylisobutylxanthine (MIX) or other cAMP elevating agents. We further show that ligands and MIX synergistically stimulated PPARd-mediated transactivation. In 3T3-L1 preadipocytes, simultaneous administration of a PPARd-selective ligand and MIX significantly enhanced the early expression of PPAR¿ and ALBP/aP2, but only modestly promoted terminal differentiation as determined by lipid accumulation. Finally, we provide evidence that synergistic activation of PPARd promotes mitotic clonal expansion in 3T3-L1 cells with or without forced expression of PPARd. In conclusion, our results suggest that PPARd may play a role in the proliferation of adipocyte precursor cells, whereas activation of endogenous PPARd in 3T3-L1 cells appears to have only minor impact on the processes leading to terminal adipocyte differentiation.

AB - The peroxisome proliferator-activated receptor ¿ (PPAR¿) is a key regulator of terminal adipocyte differentiation. PPARd is expressed in preadipocytes, but the importance of this PPAR subtype in adipogenesis has been a matter of debate. Here we present a critical evaluation of the role of PPARd in adipocyte differentiation. We demonstrate that treatment of NIH-3T3 fibroblasts overexpressing PPARd with standard adipogenic inducers led to induction of PPAR¿2 expression and terminal adipocyte differentiation in a manner that was strictly dependent on simultaneous administration of a PPARd ligand and methylisobutylxanthine (MIX) or other cAMP elevating agents. We further show that ligands and MIX synergistically stimulated PPARd-mediated transactivation. In 3T3-L1 preadipocytes, simultaneous administration of a PPARd-selective ligand and MIX significantly enhanced the early expression of PPAR¿ and ALBP/aP2, but only modestly promoted terminal differentiation as determined by lipid accumulation. Finally, we provide evidence that synergistic activation of PPARd promotes mitotic clonal expansion in 3T3-L1 cells with or without forced expression of PPARd. In conclusion, our results suggest that PPARd may play a role in the proliferation of adipocyte precursor cells, whereas activation of endogenous PPARd in 3T3-L1 cells appears to have only minor impact on the processes leading to terminal adipocyte differentiation.

U2 - 10.1074/jbc.M005567200

DO - 10.1074/jbc.M005567200

M3 - Journal article

C2 - 11069900

VL - 276

SP - 3175

EP - 3182

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -

ID: 14639694