PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
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PhenoChip : A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae. / Behrendt, Lars; Mehdi Salek, M.; Trampe, Erik L.; Fernandez, Vicente I.; Lee, Kang Soo; Kühl, Michael; Stocker, Roman.
In: Science Advances, Vol. 6, No. 36, eabb2754, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - PhenoChip
T2 - A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
AU - Behrendt, Lars
AU - Mehdi Salek, M.
AU - Trampe, Erik L.
AU - Fernandez, Vicente I.
AU - Lee, Kang Soo
AU - Kühl, Michael
AU - Stocker, Roman
PY - 2020
Y1 - 2020
N2 - Photosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience ("phenotyping") has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed "PhenoChip,"a platform for the multiparametric photophysiological characterization and selection of unicellular phenotypes under user-controlled physicochemical conditions. We used PhenoChip to expose single cells of the coral symbiont Symbiodinium to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthetic performance, and permitted the identification of single cells with elevated resilience toward rising temperature. Optical expulsion technology was used to collect single cells from PhenoChip, and their propagation revealed indications of transgenerational preservation of photosynthetic phenotypes. PhenoChip represents a versatile platform for the phenotyping of photosynthetic unicells relevant to biotechnology, ecotoxicology, and assisted evolution.
AB - Photosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience ("phenotyping") has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed "PhenoChip,"a platform for the multiparametric photophysiological characterization and selection of unicellular phenotypes under user-controlled physicochemical conditions. We used PhenoChip to expose single cells of the coral symbiont Symbiodinium to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthetic performance, and permitted the identification of single cells with elevated resilience toward rising temperature. Optical expulsion technology was used to collect single cells from PhenoChip, and their propagation revealed indications of transgenerational preservation of photosynthetic phenotypes. PhenoChip represents a versatile platform for the phenotyping of photosynthetic unicells relevant to biotechnology, ecotoxicology, and assisted evolution.
U2 - 10.1126/sciadv.abb2754
DO - 10.1126/sciadv.abb2754
M3 - Journal article
C2 - 32917592
AN - SCOPUS:85090878142
VL - 6
JO - Science advances
JF - Science advances
SN - 2375-2548
IS - 36
M1 - eabb2754
ER -
ID: 249945028