Plant YTHDF proteins are direct effectors of antiviral immunity against an N6-methyladenosine-containing RNA virus
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Plant YTHDF proteins are direct effectors of antiviral immunity against an N6-methyladenosine-containing RNA virus. / Martínez-Pérez, Mireya; Aparicio, Frederic; Arribas-Hernández, Laura; Tankmar, Mathias Due; Rennie, Sarah; von Bülow, Sören; Lindorff-Larsen, Kresten; Brodersen, Peter; Pallas, Vicente.
In: EMBO Journal, Vol. 42, No. 18, e113378, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plant YTHDF proteins are direct effectors of antiviral immunity against an N6-methyladenosine-containing RNA virus
AU - Martínez-Pérez, Mireya
AU - Aparicio, Frederic
AU - Arribas-Hernández, Laura
AU - Tankmar, Mathias Due
AU - Rennie, Sarah
AU - von Bülow, Sören
AU - Lindorff-Larsen, Kresten
AU - Brodersen, Peter
AU - Pallas, Vicente
N1 - Publisher Copyright: © 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
PY - 2023
Y1 - 2023
N2 - In virus–host interactions, nucleic acid-directed first lines of defense that allow viral clearance without compromising growth are of paramount importance. Plants use the RNA interference pathway as a basal antiviral immune system, but additional RNA-based mechanisms of defense also exist. The infectivity of a plant positive-strand RNA virus, alfalfa mosaic virus (AMV), relies on the demethylation of viral RNA by the recruitment of the cellular N6-methyladenosine (m6A) demethylase ALKBH9B, but how demethylation of viral RNA promotes AMV infection remains unknown. Here, we show that inactivation of the Arabidopsis cytoplasmic YT521-B homology domain (YTH)-containing m6A-binding proteins ECT2, ECT3, and ECT5 is sufficient to restore AMV infectivity in partially resistant alkbh9b mutants. We further show that the antiviral function of ECT2 is distinct from its previously demonstrated function in the promotion of primordial cell proliferation: an ect2 mutant carrying a small deletion in its intrinsically disordered region is partially compromised for antiviral defense but not for developmental functions. These results indicate that the m6A-YTHDF axis constitutes a novel branch of basal antiviral immunity in plants.
AB - In virus–host interactions, nucleic acid-directed first lines of defense that allow viral clearance without compromising growth are of paramount importance. Plants use the RNA interference pathway as a basal antiviral immune system, but additional RNA-based mechanisms of defense also exist. The infectivity of a plant positive-strand RNA virus, alfalfa mosaic virus (AMV), relies on the demethylation of viral RNA by the recruitment of the cellular N6-methyladenosine (m6A) demethylase ALKBH9B, but how demethylation of viral RNA promotes AMV infection remains unknown. Here, we show that inactivation of the Arabidopsis cytoplasmic YT521-B homology domain (YTH)-containing m6A-binding proteins ECT2, ECT3, and ECT5 is sufficient to restore AMV infectivity in partially resistant alkbh9b mutants. We further show that the antiviral function of ECT2 is distinct from its previously demonstrated function in the promotion of primordial cell proliferation: an ect2 mutant carrying a small deletion in its intrinsically disordered region is partially compromised for antiviral defense but not for developmental functions. These results indicate that the m6A-YTHDF axis constitutes a novel branch of basal antiviral immunity in plants.
KW - ECTs
KW - IDRs
KW - N6-methyladenosine (mA)
KW - plant–virus interaction
KW - RNA methylation
U2 - 10.15252/embj.2022113378
DO - 10.15252/embj.2022113378
M3 - Journal article
C2 - 37431920
AN - SCOPUS:85165029189
VL - 42
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 18
M1 - e113378
ER -
ID: 362746395