PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters

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PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters. / Preker, Pascal; Almvig, Kristina; Christensen, Marianne S; Valen, Eivind; Mapendano, Christophe Kamungu; Sandelin, Albin Gustav; Jensen, Torben Heick.

In: Nucleic Acids Research, 19.05.2011.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Preker, P, Almvig, K, Christensen, MS, Valen, E, Mapendano, CK, Sandelin, AG & Jensen, TH 2011, 'PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters', Nucleic Acids Research. https://doi.org/10.1093/nar/gkr370

APA

Preker, P., Almvig, K., Christensen, M. S., Valen, E., Mapendano, C. K., Sandelin, A. G., & Jensen, T. H. (2011). PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters. Nucleic Acids Research. https://doi.org/10.1093/nar/gkr370

Vancouver

Preker P, Almvig K, Christensen MS, Valen E, Mapendano CK, Sandelin AG et al. PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters. Nucleic Acids Research. 2011 May 19. https://doi.org/10.1093/nar/gkr370

Author

Preker, Pascal ; Almvig, Kristina ; Christensen, Marianne S ; Valen, Eivind ; Mapendano, Christophe Kamungu ; Sandelin, Albin Gustav ; Jensen, Torben Heick. / PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters. In: Nucleic Acids Research. 2011.

Bibtex

@article{5cef74e43725405a8ad27e87a87293bc,
title = "PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters",
abstract = "PROMoter uPstream Transcripts (PROMPTs) were identified as a new class of human RNAs, which are heterologous in length and produced only upstream of the promoters of active protein-coding genes. Here, we show that PROMPTs carry 3'-adenosine tails and 5'-cap structures. However, unlike mRNAs, PROMPTs are largely nuclear and rapidly turned over by the RNA exosome. PROMPT-transcribing DNA is occupied by RNA polymerase II (RNAPII) complexes with serine 2 phosphorylated C-terminal domains (CTDs), mimicking that of the associated genic region. Thus, the inefficient elongation capacity of PROMPT transcription cannot solely be assigned to poor CTD phosphorylation. Conditions that reduce gene transcription increase RNAPII occupancy of the upstream PROMPT region, suggesting that they reside in a common transcription compartment. Surprisingly, gene promoters that are actively transcribed by RNAPI or RNAPIII also produce PROMPTs that are targeted by the exosome. RNAPIII PROMPTs bear hallmarks of RNAPII promoter-associated RNAs, explaining the physical presence of RNAPII upstream of many RNAPIII-transcribed genes. We propose that RNAPII activity upstream gene promoters are wide-spread and integral to the act of gene transcription.",
author = "Pascal Preker and Kristina Almvig and Christensen, {Marianne S} and Eivind Valen and Mapendano, {Christophe Kamungu} and Sandelin, {Albin Gustav} and Jensen, {Torben Heick}",
year = "2011",
month = may,
day = "19",
doi = "10.1093/nar/gkr370",
language = "English",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - PROMoter uPstream Transcripts share characteristics with mRNAs and are produced upstream of all three major types of mammalian promoters

AU - Preker, Pascal

AU - Almvig, Kristina

AU - Christensen, Marianne S

AU - Valen, Eivind

AU - Mapendano, Christophe Kamungu

AU - Sandelin, Albin Gustav

AU - Jensen, Torben Heick

PY - 2011/5/19

Y1 - 2011/5/19

N2 - PROMoter uPstream Transcripts (PROMPTs) were identified as a new class of human RNAs, which are heterologous in length and produced only upstream of the promoters of active protein-coding genes. Here, we show that PROMPTs carry 3'-adenosine tails and 5'-cap structures. However, unlike mRNAs, PROMPTs are largely nuclear and rapidly turned over by the RNA exosome. PROMPT-transcribing DNA is occupied by RNA polymerase II (RNAPII) complexes with serine 2 phosphorylated C-terminal domains (CTDs), mimicking that of the associated genic region. Thus, the inefficient elongation capacity of PROMPT transcription cannot solely be assigned to poor CTD phosphorylation. Conditions that reduce gene transcription increase RNAPII occupancy of the upstream PROMPT region, suggesting that they reside in a common transcription compartment. Surprisingly, gene promoters that are actively transcribed by RNAPI or RNAPIII also produce PROMPTs that are targeted by the exosome. RNAPIII PROMPTs bear hallmarks of RNAPII promoter-associated RNAs, explaining the physical presence of RNAPII upstream of many RNAPIII-transcribed genes. We propose that RNAPII activity upstream gene promoters are wide-spread and integral to the act of gene transcription.

AB - PROMoter uPstream Transcripts (PROMPTs) were identified as a new class of human RNAs, which are heterologous in length and produced only upstream of the promoters of active protein-coding genes. Here, we show that PROMPTs carry 3'-adenosine tails and 5'-cap structures. However, unlike mRNAs, PROMPTs are largely nuclear and rapidly turned over by the RNA exosome. PROMPT-transcribing DNA is occupied by RNA polymerase II (RNAPII) complexes with serine 2 phosphorylated C-terminal domains (CTDs), mimicking that of the associated genic region. Thus, the inefficient elongation capacity of PROMPT transcription cannot solely be assigned to poor CTD phosphorylation. Conditions that reduce gene transcription increase RNAPII occupancy of the upstream PROMPT region, suggesting that they reside in a common transcription compartment. Surprisingly, gene promoters that are actively transcribed by RNAPI or RNAPIII also produce PROMPTs that are targeted by the exosome. RNAPIII PROMPTs bear hallmarks of RNAPII promoter-associated RNAs, explaining the physical presence of RNAPII upstream of many RNAPIII-transcribed genes. We propose that RNAPII activity upstream gene promoters are wide-spread and integral to the act of gene transcription.

U2 - 10.1093/nar/gkr370

DO - 10.1093/nar/gkr370

M3 - Journal article

C2 - 21596787

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

ER -

ID: 33535069